A label-free and high sensitive aptamer biosensor based on hyperbranched polyester microspheres for thrombin detection
文献类型： 外文期刊
作者： Sun, Chong 1 ; Han, Qiaorong 1 ; Wang, Daoying 2 ; Xu, Weimin 2 ; Wang, Weijuan 1 ; Zhao, Wenbo 1 ; Zhou, Min 3 ;
作者机构： 1.Nanjing Normal Univ, Coll Chem & Mat Sci, Biomed Funct Mat Collaborat Innovat Ctr, Jiangsu Key Lab Biofunct Mat, Nanjing 210023, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing 270014, Jiangsu, Peoples R China
3.Nanjing Univ, Sch Med, Drum Tower Hosp, Dept Vasc Surg, Nanjing 270008, Jiangsu, Peoples R China
关键词： Hyperbranched polyester microspheres;Whole blood;Antibiofouling;Aptamer biosensor;Thrombin
期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.558； 五年影响因子：6.228 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： In this paper, we have synthesized hyperbranched polyester microspheres with carboxylic acid functional groups (HBPE-CA) and developed a label-free electrochemical aptamer biosensor using thrombin-binding aptamer (TBA) as receptor for the measurement of thrombin in whole blood. The indium tin oxide (ITO) electrode surface modified with HBPE-CA microspheres was grafted with TBA, which has excellent binding affinity and selectivity for thrombin. Binding of the thrombin at the modified ITO electrode surface greatly restrained access of electrons for a redox probe of [Fe(CN)6]~(3-/4-). Moreover, the aptamer biosensor could be used for detection of thrombin in whole blood, a wide detection range (10 fiVHOO nM) and a detection limit on the order of 0.90 fM were demonstrated. Control experiments were also carried out by using bull serum albumin (BSA) and lysozyme in the absence of thrombin. The good stability and repeatability of this aptamer biosensor were also proved. We expect that this demonstration will lead to the development of highly sensitive label-free sensors based on aptamer with lower cost than current technology. The integration of the technologies, which include anticoagulant, sensor and nanoscience, will bring significant input to high-performance biosensors relevant to diagnostics and therapy of interest for human health.
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