Isolation and characterization of a cryptogein-like gene from drought and salt-treated Alternanthera philoxeroides roots
文献类型： 外文期刊
作者： Gao, Jianming 1 ; Li, Jinzhi 2 ; Xiao, Qiang 3 ; Luo, Caibo 3 ; Zhang, Shiqing 1 ; Chen, Helong 1 ; Yi, Kexian 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Minist Agr, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Peoples R China
2.Taizhou Univ, Sch Life Sci, Taizhou 318000, Zhejiang, Peoples R China
3.HUST, China UK HUST RRes Genet Engn & Genom Joint Lab, Wuhan 430074, Peoples R China
期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）
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收录情况： SCI
摘要： Alligator weed, Alternanthera philoxeroides (Mart.) Grisb, is an amphibious plant with long thick fleshy roots that develop from adventitious roots under drought conditions. To clone differentially-expressed genes from the roots of A. philoxeroides we applied both mRNA differential display and rapid amplification of cDNA ends techniques. A cryptogein-like gene of A. philoxeroides, designated as ApCL, was cloned. On the basis of semi-quantitative RT-PCR analysis results, we demonstrated that the ApCL gene was upregulated under drought and salt stress conditions. After ApCL was transferred to Pichia pastoris GS115 and its resistance to drought and salt then increased by > 100 %. Therefore, the ApCL gene of A. philoxeroides was likely involved in drought and salt tolerance responses
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