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Functional Characterization of 5-Enopyruvylshikimate-3-Phosphate Synthase from Alkaliphilus metalliredigens in Transgenic Arabidopsis

文献类型： 外文期刊

作者： Xing, Xiao-Juan 1 ; Tian, Yong-Sheng 2 ; Peng, Ri-He 2 ; Xu, Jing 2 ; Zhao, Wei 2 ; Yao, Quan-Hong 2 ; Sun, Sheng 1 ;

作者机构： 1.Shanxi Agr Univ, Coll Hort, Taigu 030801, Peoples R China

2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China

关键词： 5-Enopyruvylshikimate-3-phosphate synthase;Alkaliphilus metalliredigens;glyphosate;transgenic Arabidopsis

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

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收录情况： SCI

摘要： Although a large number of AroA enzymes (EPSPS: 5-enopyruvylshikimate-3-phosphate synthase) have been identified, cloned, and tested for glyphosate resistance, only two AroA variants, derived from Agrobacterium tumefaciens strain CP4 and Zea mays, have been utilized to produce the commercial glyphosate-resistant crops. Here, we have used a PCR-based two- step DNA synthesis method to synthesize an aroA gene （aroA_(A. metalliredigens)) from Alkaliphilus metalliredigens, encoding a new EPSPS. Furthermore, transgenic Arabidopsis with the new aroAA metalliredigens gene was obtained to confirm the potential of the novel aroA gene in developing glyphosate-resistant crops.

  • 相关文献

[1]Functional characterization of Class II 5-enopyruvylshikimate-3-phosphate synthase from Halothermothrix orenii H168 in Escherichia coli and transgenic Arabidopsis. Tian, Yong-Sheng,Xu, Jing,Xiong, Ai-Sheng,Zhao, Wei,Gao, Feng,Fu, Xiao-Yan,Peng, Ri-He,Yao, Quan-Hong.

[2]Novel Glyphosate-Resistant aroA Gene from Paracoccus denitrificans in Transgenic Arabidopsis. Tian, Y. -S.,Xiong, A. -S.,Tian, Y. -S.,Yao, Q. -H.,Peng, R. -H.,Xu, J.,Tian, Y. -S.,Xing, X. -J..

[3]Isolation from Proteus mirabilis of a novel class I 5-enopyruvylshikimate-3-phosphate synthase with glyphosate tolerance in transgenic Arabidopsis thaliana. Tian, Yong-Sheng,Jin, Xiao-Fen,Xu, Jing,Han, Jing,Zhao, Wei,Fu, Xiao-Yan,Peng, Ri-He,Wang, Rong-Tan,Yao, Quan-Hong.

[4]Phytoremediation of triphenylmethane dyes by overexpressing a Citrobacter sp triphenylmethane reductase in transgenic Arabidopsis. Fu, Xiao-Yan,Zhao, Wei,Xiong, Ai-Sheng,Tian, Yong-Sheng,Zhu, Bo,Peng, Ri-He,Yao, Quan-Hong. 2013

[5]Phytoremediation of 2,4,6-trinitrotoluene by Arabidopsis plants expressing a NAD(P)H-flavin nitroreductase from Enterobacter cloacae. You, Shuang-Hong,Zhu, Bo,Han, Hong-Juan,Wang, Bo,Peng, Ri-He,Yao, Quan-Hong.

[6]Functional Characterization of aroA from Rhizobium leguminosarum with Significant Glyphosate Tolerance in Transgenic Arabidopsis. Han, Jing,Tian, Yong-Sheng,Xu, Jing,Wang, Li-Juan,Wang, Bo,Peng, Ri-He,Yao, Quan-Hong.

[7]Enhancement of naphthalene tolerance in transgenic Arabidopsis plants overexpressing the ferredoxin-like protein (ADI1) from rice. Fu, Xiao-Yan,Zhu, Bo,Han, Hong-Juan,Zhao, Wei,Tian, Yong-Sheng,Peng, Ri-He,Yao, Quan-Hong.

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