Functional Characterization of 5-Enopyruvylshikimate-3-Phosphate Synthase from Alkaliphilus metalliredigens in Transgenic Arabidopsis
文献类型： 外文期刊
作者： Xing, Xiao-Juan 1 ; Tian, Yong-Sheng 2 ; Peng, Ri-He 2 ; Xu, Jing 2 ; Zhao, Wei 2 ; Yao, Quan-Hong 2 ; Sun, Sheng 1 ;
作者机构： 1.Shanxi Agr Univ, Coll Hort, Taigu 030801, Peoples R China
2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
关键词： 5-Enopyruvylshikimate-3-phosphate synthase;Alkaliphilus metalliredigens;glyphosate;transgenic Arabidopsis
期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）
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收录情况： SCI
摘要： Although a large number of AroA enzymes (EPSPS: 5-enopyruvylshikimate-3-phosphate synthase) have been identified, cloned, and tested for glyphosate resistance, only two AroA variants, derived from Agrobacterium tumefaciens strain CP4 and Zea mays, have been utilized to produce the commercial glyphosate-resistant crops. Here, we have used a PCR-based two- step DNA synthesis method to synthesize an aroA gene （aroA_(A. metalliredigens)) from Alkaliphilus metalliredigens, encoding a new EPSPS. Furthermore, transgenic Arabidopsis with the new aroAA metalliredigens gene was obtained to confirm the potential of the novel aroA gene in developing glyphosate-resistant crops.
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