您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Development of highly sensitive electrochemical genosensor based on multiwalled carbon nanotubes-chitosan-bismuth and lead sulfide nanoparticles for the detection of pathogenic Aeromonas

文献类型： 外文期刊

作者： Fernandes, Antonio Maximiano 1 ; Abdalhai, Mandour H. 1 ; Ji, Jian 1 ; Xi, Bing-Wen 2 ; Xie, Jun; Sun, Jiadi 1 ; No 1 ;

作者机构： 1.Jiangnan Univ, Synerget Innovat Ctr Food Safety & Nutr, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 7214122, Jiangsu, Peoples R China

2.Chinese Acad Fishery Sci, Minist Agr, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & G

关键词： Electrochemical biosensor;Aeromonas;Lead sulfide nanoparticles;Foodborne pathogen

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this paper, we reported the construction of new high sensitive electrochemical genosensor based on multiwalled carbon nanotubes-chitosan-bismuth complex (MWCNT-Chi-Bi) and lead sulfide nanopartides for the detection of pathogenic Aeromonas. Lead sulfide nanoparticles capped with 5'-(NH2) oligonucleotides thought amide bond was used as signalizing probe DNA (sz-DNA) and thiol-modified oligonucleotides sequence was used as fixing probe DNA (fDNA). The two probes hybridize with target Aeromonas DNA (tDNA) sequence (fDNA-tDNA-szDNA). The signal of hybridization is detected by differential pulse voltammetry (DPV) after electrodeposition of released lead nanoparticles (PbS) from sz-DNA on the surface of glass carbon electrode decorated with MWCNT-Chi-Bi, which improves the deposition and traducing electrical signal. The optimization of incubation time, hybridization temperature, deposition potential, deposition time and the specificity of the probes were investigated. Our results showed the highest sensibility to detect the target gene when compared with related biosensors and polymerase chain reaction (PCR). The detection limit for this biosensor was 1.0 x 10(-14) M. We could detect lower than 10(2) CFU mL(-1) of Aeromonas in spiked tap water. This method is rapid and sensitive for the detection of pathogenic bacteria and would become a potential application in biomedical diagnosis, food safety and environmental monitoring. (C) 2014 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Multi-Drug Resistance Mediated by Class 1 Integrons in Aeromonas Isolated from Farmed Freshwater Animals. Deng, Yuting,Wu, Yali,Jiang, Lan,Tan, Aiping,Zhang, Ruiquan,Luo, Li,Wu, Yali. 2016

[2]PdS Nanoparticle Label Based DNA Biosensor for Rapid Detection of Mercury (II). Wu, LiDong,Ding, YanHua,Hong, Nian,Cheng, Lin,Fan, Hao. 2014

[3]A Novel Electrochemical Biosensor Based on DNA for Rapid and Selective Detection of Cadmium. Qu, Jialin,Wu, Lidong,Liu, Huan,Li, Jincheng,Lv, Haiyan,Fu, Xiaochen,Song, Yi. 2015

[4]A Novel Tyrosinase Biosensor based on Graphene and Co3O4 Nanocomposite Materials for Rapid Determining Catechol. Liang, Kehong,Qin, Yuchang,Fu, Xiaochen,Wu, Lidong,Song, Yi. 2016

[5]3D metal-organic framework as highly efficient biosensing platform for ultrasensitive and rapid detection of bisphenol A. Wang, Xue,Lu, Xianbo,Chen, Jiping,Wang, Xue,Wu, Lidong.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台