The expression of the multiple splice variants of AQP8 in porcine testes at different developmental stages
文献类型： 外文期刊
作者： Gao, Y. 1 ; Deng, Q. 1 ; Zhang, Y. 1 ; Zhang, S. 2 ; Zhu, Y. 1 ; Zhang, J. 1 ;
作者机构： 1.Jilin Univ, Lab Anim Ctr, Coll Anim Sci, Changchun 130062, Peoples R China
2.Jilin Acad Agr Sci, Branch Anim Husb, Gongzhuling 136100, Peoples R China
关键词： Alternative splicing;AQP8;Porcine;RT-PCR;Testis
期刊名称：JOURNAL OF APPLIED GENETICS （ 影响因子：3.24； 五年影响因子：2.756 ）
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收录情况： SCI
摘要： Aquaporin 8 (AQP8) may be involved in cytoplasmic condensation and fluid formation in the vas deferens during the process of spermiogenesis. In the present study, using reverse transcription polymerase chain reaction (RT-PCR) combined with DNA sequencing technology, the splice isoforms of AQP8 post-transcriptionally formed by alternative splicing in porcine testis at various developmental stages were analysed, and the differences in the amino acid sequences encoded by the splice isoforms were compared. The results showed that a total of three splice isoforms of AQP8 mRNA, including full-length mRNA, were produced via alternative splicing in porcine testes at the four developmental stages. Because the number of bases removed during splicing was not an integer multiple of 3, the amino acid sequence and protein structure of AQP8 were significantly altered after alternative splicing.
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