Genome-wide analysis of terpene synthases in soybean: Functional characterization of GmTPS3
文献类型： 外文期刊
作者： Liu, Jianyu 2 ; Huang, Fang 1 ; Wang, Xia 1 ; Zhang, Man 3 ; Zheng, Rui 4 ; Wang, Jiao 1 ; Yu, Deyue 1 ;
作者机构： 1.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Natl Ctr Soybean Improvement, Nanjing 210095, Jiangsu, Peoples R China
2.Shanghai Acad Agr Sci, Inst Edible Fungi, Natl Engn Res Ctr Edible Fungi, Shanghai 201403, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Nanjing 210014, Jiangsu, Peoples R China
4.Ningxia Univ, Coll Life Sci, Yinchuan 750021, Peoples R China
关键词： Cotton leafworms;FPP;GC-MS;Geraniol synthase;GPP;Insect resistance;QRT-PCR;Soybean;Terpene synthase
期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）
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年卷期：
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收录情况： SCI
摘要： Terpenes (terpenoids or isoprenoids) constitute a large class of plant natural products and play numerous functional roles in primary and secondary metabolism as well as inecological interactions. This study presents a genomic analysis of 23 putative soybean (Glycine max) terpene synthase genes (GmTPSs) distributed over 10 of 20 chromosomes. The GmTPSs are grouped into six types based on gene architecture and sequence identity. Sequence alignment indicates that most GmTPSs contain the conserved aspartate-rich DDX2D motif, and two clades encoded by TPS-a and TPS-b contain variations of an arginine-rich RRX8W motif. Quantitative real-time PCR analysis demonstrated that GmTPSs were predominantly expressed in reproductive organs. Heterologous expression followed by enzymatic assay suggested that GmTPS3 functions as a geraniol synthase. We also generated transgenic tobacco plants ectopically expressing GmTPS3. In dual-choice feeding-preference and force-feeding assays, the transgenic tobacco lines expressing GmTPS3 exhibited enhanced resistance to cotton leafworms and an increased level of geraniol. Taken together, these data provide a comprehensive understanding of the TPS family in soybeans and suggest a promising approach to engineering transgenic plants with enhanced insect resistance.
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