您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

High resolution melting curve analysis as a new tool for rapid identification of canine parvovirus type 2 strains

文献类型： 外文期刊

作者： Bingga, Gali 1 ; Liu, Zhicheng 2 ; Zhang, Jianfeng 2 ; Zhu, Yujun 3 ; Lin, Lifeng 2 ; Ding, Shuangyang 1 ; Guo, Pengju 4 ;

作者机构： 1.China Agr Univ, Coll Vet Med, Dept Vet Pharmacol & Toxicol, Beijing 100094, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Guangdong, Peoples R China

3.Guangzhou Bortzi Biotech Ltd, Guangzhou, Guangdong, Peoples R China

4.Guangdong Lab Anim Monitoring Inst, Guangdong Key Lab Lab Anim, Guangzhou, Guangdong, Peoples R China

关键词： PCR;Canine parvovirus;VP2 gene;High resolution melting curve analysis;Heteroduplex

期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A high resolution melting (HRM) curve method was developed to identify canine parvovirus type 2 (CPV-2) strains by nested PCR. Two sets of primers, CPV-426F/426R and CPV-87R/87F, were designed that amplified a 52 bp and 53 bp product from the viral VP2 capsid gene. The region amplified by CPV-426F/426R included the A4062G and T4064A mutations in CPV-2a, CPV-2b and CPV-2c. The region amplified by CPV-87F/87R included the A3045T mutation in the vaccine strains of CPV-2 and CPV-2a, CPV-2b and CPV-2c. Faecal samples were obtained from 30 dogs that were CPV antigen-positive. The DNA was isolated from the faecal samples and PCR-amplified using the two sets of primers, and genotyped by HRM curve analysis. The PCR-HRM assay was able to distinguish single nucleotide polymorphisms between CPV-2a, CPV-2b and CPV-2c using CPV-426F/426R. CPV-2a was distinguished from CPV-2b and CPV-2c by differences in the melting temperature. CPV-2b and CPV-2c could be distinguished based on the shape of the melting curve after generating heteroduplexes using a CPV-2b reference sample. The vaccine strains of CPV-2 were identified using CPV-87F/87R. Conventional methods for genotyping CPV strains are labor intensive, expensive or time consuming; the present PCR-based HRM assay might be an attractive alternative. (C) 2014 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]猪Ⅱ型圆环病毒ORF1、ORF2基因串联重组腺病毒的构建. 祝卫国,西北农林科技大学动物科技学院,宋长绪,王贵平,杨增歧,王旭荣,黄忠,张春红. (Mis

[2]Development and application of a loop-mediated isothermal amplification method for rapid detection of Haemophilus parasuis. Zhang, Jian-Min,Xu, Cheng-Gang,Guo, Li-Li,Zhang, Bin,Li, Jing-Yi,Chen, Ji-Dang,Fan, Hui-Ying,Liao, Ming,Shen, Hai-Yan. 2011

[3]Rapid Identification of Peanut Hybrids with SSR-PCR. Hong Yanbin,Li Xingyu,Huang Shangzhi,Hong Yanbin,Chen Xiaoping,Liang Xuanqiang,Liu Hong. 2015

[4]粤东地区鸡球虫病流行病学调查. 余志会,方肆云,刘文俊,戚南山,李娟,吴彩艳,林栩慧,吕敏娜,蔡海明,胡俊菁,宋勇乐,陈祥杰,朱易斌,尹理君,张健騑,廖申权,孙铭飞. 2024

[5]广州市部分区域猫泛白细胞减少症流行现状调查. 温肖会,邱杰,吕殿红,翟少伦,魏文康. 2016

[6]中国海南野番茄上的一种新病毒-野番茄花叶病毒. 张真,章绍延,余乃通,王健华,龚殿. 2015

[7]猪链球菌2型和9型广东分离株的病原特性. 余炜烈,李春玲,王贵平,陆承平. 2007

[8]一株血清7型致病性猪链球菌的分离鉴定. 徐敏,刘永刚,石文达,武嘉男,刘鹤,何玉利,姜成刚,韩梓峰,蔡雪辉. 2011

[9]鸭瘟病毒的PCR检测及其产物分析. 陈建君,张毓金,杨增岐,宋长绪. 2003

[10]吉林省2006-2010年羊传染性脓疱病的流行病学调查及分析. 郭良兴,赵魁,赵广海,贺文琦,袁洁,陆慧君,王改丽,苏高莉,高丰. 2011

[11]一种简单快速的DNA模板制备方法. 罗文永,李晓方,肖昕,毛兴学,刘彦卓. 2002

[12]番鸭和鹅细小病毒PCR鉴别方法的建立. 季芳,张毓金,杨增岐,宋长绪. 2003

[13]应用PCR及Nested-PCR技术检测柑橘黄龙病病原研究. 丁芳,易干军,王国平. 2004

[14]猪链球菌扁桃体分离株的毒力因子分布特征与致病性. 李春玲,余炜烈,王贵平,贾爱卿,孙俊颖,陆承平. 2009

[15]鸭瘟病毒UL35基因PCR方法的建立与初步应用. 董嘉文,孙敏华,吕殿红,胡奇林. 2012

[16]猪附红细胞体PCR-微孔板杂交-酶免疫检测方法的研究. 张浩吉,谢明权,张健騑,蒲文珺,覃宗华,蔡建平,王政富,顾万军. 2007

[17]粤北地区鸡球虫病流行病学调查. 殷熙娟,王定爱,刘文俊,戚南山,李娟,吴彩艳,吕敏娜,蔡海明,胡俊菁,林栩慧,宋勇乐,陈祥杰,朱易斌,尹理君,张健騑,廖申权,孙铭飞. 2023

[18]猪链球菌及其血清型的PCR检测. 臧莹安,胡波,卢頔,宋帅,李淼,李春玲. 2011

[19]副猪嗜血杆菌和猪链球菌双重PCR方法的建立与应用. 贾爱卿,李春玲,王贵平,杨冬霞,何丽丽,王金生. 2009

[20]猪Torque teno病毒广东株的PCR检测和序列分析. 黄元,徐敏,陈少勤,王晓虎,向华,张健騑. 2012

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台