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Affinity chromatography revealed insights into unique functionality of two 14-3-3 protein species in developing maize kernels

文献类型： 外文期刊

作者： Dou, Yao 2 ; Liu, Xiangguo 1 ; Yin, Yuejia 1 ; Han, Siping 1 ; Lu, Yang 3 ; Liu, Yang 1 ; Hao, Dongyun 1 ;

作者机构： 1.Jilin Acad Agr Sci, Inst Agr Biotechnol, Changchun 130124, Peoples R China

2.Jilin Univ, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, Peoples R China

3.Jilin Acad Agr Sci, Inst Plant Protect, Changchun 130124, Peoples R China

关键词： 14-3-3 protein species;Functional characterization;Zea mays;Affinity chromatography;Kernel development

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：4.044； 五年影响因子：4.02 ）

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收录情况： SCI

摘要： The 14-3-3 proteins are a group of regulatory proteins of divergent functions in plants. However, little is known about their roles in maize kernel development. Using publically available gene expression profiling data, we found that two 14-3-3 species genes, zmgf14-4 and zmgf14-6, exhibited prominent expression profiles over other 14-3-3 protein genes during maize kernel development. More than 5000 transcripts of these two genes were identified accounting for about 1/10 of the total transcripts of genes correlating to maize kernel development. We constructed a proteomics pipeline based on the affinity chromatography, in combination with 2-DE and LC-MS/MS technologies to identify the specific client proteins of the two proteins for their functional characterization. Consequently, we identified 77 specific client proteins from the developing kernels of the inbred maize B73. More than 60% of the client proteins were commonly affinity-identified by the two 14-3-3 species and are predicted to be implicated in the fundamental functions of metabolism, protein destination and storage. In addition, we found ZmGF14-4 specifically bound to the disease- or defense-relating proteins, whilst ZmGF14-6 tended to interact with the proteins involving metabolism and cell structure. Our findings provide primary insights into the functional roles of 14-3-3 proteins in maize kernel development.

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