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Quantitative proteomics and transcriptomics reveal key metabolic processes associated with cotton fiber initiation

文献类型: 外文期刊

作者: Wang, Xu-Chu 1 ; Li, Qin 1 ; Jin, Xiang 2 ; Xiao, Guang-Hui 1 ; Liu, Gao-Jun 1 ; Liu, Nin-Jing 1 ; Qin, Yong-Mei 1 ;

作者机构: 1.Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Hainan, Peoples R China

关键词: Cotton fiber initiation;Gossypium hirsutum;Hydroxyacyl-CoA dehydratase;iTRAQ;RNA-sequencing;Very long-chain fatty acid

期刊名称:JOURNAL OF PROTEOMICS ( 影响因子:4.044; 五年影响因子:4.02 )

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收录情况: SCI

摘要: An iTRAQ-based proteomics of ovules from the upland cotton species Gossypiurn hirsutum and its fuzzless-lintless mutant was performed, and finally 2729 proteins that preferentially accumulated at anthesis in wild-type ovules were identified. We confirmed that the gene expression levels of 2005 among these proteins also increased by performing an RNA sequencing transcriptomics. Expression of proteins involved in carboxylic acid metabolism, small-molecule metabolic processes, hormone regulation, and lipid metabolism was significantly enhanced in wild-type ovules. Quantitative real-time PCR verified the increased expression of 26 genes involved in these processes. Cotton 3-hydroxyacyl-CoA dehydratase (GhPAS2) catalyzing the third reaction of very long-chain fatty acid (VLCFA) biosynthesis, accumulated at anthesis in wild-type ovules. Heterogeneous expression of GhPAS2 restored viability to the Saccharomyces cereuisiae haploid psh1-deletion strain deficient in PAS2 activity. Application of VLCFA biosynthesis inhibitor acetochlor (2-chloro-N-[ethoxymethyl]-N-[2-ethyl-6-methyl-phenl]-acetamide; ACE) and gibberellic acid to the unfertilized cotton ovules significantly suppressed fiber cell protrusion. In this study, the profiling of gene expression at both transcriptome and proteome levels provides new insights into cotton fiber cell initiation.

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