Genome-wide investigation and expression profiling of APX gene family in Gossypium hirsutum provide new insights in redox homeostasis maintenance during different fiber development stages
文献类型: 外文期刊
作者: Tao, Chengcheng 1 ; Jin, Xiang 1 ; Zhu, Liping 1 ; Xie, Quanliang 1 ; Wang, Xuchu 1 ; Li, Hongbin 1 ;
作者机构: 1.Shihezi Univ, Coll Life Sci, Key Lab Agrobiotechnol, Shihezi, Xinjiang, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou, Hainan, Peoples R China
关键词: Ascorbate peroxidase; Expression profiling; Redox homeostasis; Gossypium hirsutum; Cotton fiber
期刊名称:MOLECULAR GENETICS AND GENOMICS ( 影响因子:3.291; 五年影响因子:3.257 )
ISSN: 1617-4615
年卷期: 2018 年 293 卷 3 期
页码:
收录情况: SCI
摘要: Ascorbate peroxidase (APX) is a member of heme-containing peroxidases which catalyze the H2O2-dependent oxidation of a wide range of substrates in plants and animals. As is known, H2O2 acts as a signaling molecule in the regulation of fiber development. Our previous work reported that ascorbate peroxidase 1 (GhAPX1) was important for cotton fiber elongation. However, knowledge about APX gene family members and their evolutionary and functional characteristics in cotton is limited. Here, we report 26 GhAPX genes by genome-wide investigation of tetraploid cotton Gossypium hirsutum. Phylogenetic and gene structure analyses classified these APX members into five clades and syntenic analysis suggested two duplication events. Expression profiling of the 26 APXs revealed that ten members are expressed in cotton fibers. Notably, GhAPX10A, GhAPX10D, GhAPX12A, and GhAPX12D showed high expression levels in 30-day fiber, while GhAPX1A/D, GhAPX3A/D, and GhAPX6A/D showed very low expression levels. The enzyme activity and H2O2 content assays revealed that cotton fiber kept high enzyme activity and the lowest H2O2 level in 30-day fibers, indicating that other than GhAPX1, the newly reported APX members are responsible for the reactive oxygen species homeostasis in the cotton fiber maturation stages. Expression profiling of ten fiber-expressed APXs after phytohormone treatments revealed their regulation patterns by different stimuli, suggesting that GhAPX1, GhAPX12A, and GhAPX12D are responsible to most phytohormone treatments. Our data provided evolutionary and functional information of GhAPX gene family members and revealed that different members are responsible to redox homeostasis during different cotton fiber development stages.
- 相关文献
作者其他论文 更多>>
-
Genomic insight into domestication of rubber tree
作者:Chao, Jinquan;Wu, Shaohua;Shi, Minjing;Yang, Shuguang;Zhang, Shixin;Deng, Xiaomin;Gao, Xinsheng;Zhang, Xiaofei;Hu, Yanshi;Zeng, Xia;Li, Weiguo;Tian, Wei-Min;Chao, Jinquan;Wu, Shaohua;Shi, Minjing;Yang, Shuguang;Zhang, Shixin;Deng, Xiaomin;Huang, Xiao;Gao, Xinsheng;Zhang, Xiaofei;Hu, Yanshi;Zeng, Xia;Li, Weiguo;Wu, Jilin;Hao, Bingzhong;Tian, Wei-Min;Chao, Jinquan;Wu, Shaohua;Shi, Minjing;Yang, Shuguang;Zhang, Shixin;Li, Yan;Deng, Xiaomin;Huang, Xiao;Gao, Xinsheng;Zhang, Xiaofei;Hu, Yanshi;Zeng, Xia;Li, Weiguo;Wu, Jilin;Hao, Bingzhong;Tian, Wei-Min;Xu, Xia;Du, Huilong;Fan, Xiuli;Hai, Chunyan;Kou, Liquan;Yu, Hong;Li, Jiayang;Liang, Chengzhi;Gao, Qiang;Zhang, Liangsheng;Gao, Qiang;Du, Huilong;Du, Huilong;Fan, Xiuli;Hai, Chunyan;Li, Jiayang;Liang, Chengzhi;Gao, Bin;Guo, Dong;Peng, Shiqing;Zhang, Jiao;Wang, Zhiwei;Li, Yan;Xue, Wenbo;Xu, Jiang;Wang, Xuchu;Li, Jiayang
关键词:
-
An efficient Agrobacterium tumefaciens-mediated transformation system of Taraxacum kok-saghyz Rodin (Russian dandelion)
作者:Peng, Cunzhi;Chang, Lili;Wang, Dan;Zheng, Xingmei;Tong, Zheng;Peng, Cunzhi;Chang, Lili;Wang, Dan;Zheng, Xingmei;Tong, Zheng;Xie, Quanliang;Xu, Bingqiang
关键词:Taraxacum kok-saghyz Rodin; Genetic transformation system; Rubber elongation factor; Rubber-yielding plant
-
Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex
作者:Yu, Li;Yuan, Boxuan;Xie, Quanliang;Wang, Xuchu;Yu, Li;Yuan, Boxuan;Wang, Lingling;Ding, Guohua;Souleymane, Ousmane Ahmat;Zhang, Xueyan;Wang, Xuchu;Sun, Yong
关键词:comparative proteomics; Hevea brasiliensis; Hydroxymethylglutaryl-CoA synthase; glycosylated proteins; natural rubber biosynthesis; rubber latex
-
Proteomic Landscape Has Revealed Small Rubber Particles Are Crucial Rubber Biosynthetic Machines for Ethylene-Stimulation in Natural Rubber Production
作者:Wang, Dan;Xie, Quanliang;Yu, Li;Zhang, Xueyan;Yuan, Boxuan;Jin, Xiang;Wang, Xuchu;Wang, Dan;Tong, Zheng;Chang, Lili;Jin, Xiang;Wang, Xuchu;Xie, Quanliang;Yu, Li;Yuan, Boxuan;Li, Hongbin;Wang, Xuchu;Sun, Yong;He, Peng;Dong, Yiyang;Montoro, Pascal
关键词:Hevea brasiliensis; natural rubber biosynthesis; protein phosphorylation; quantitative proteomics; small rubber particle
-
Proteomic Landscape of the Mature Roots in a Rubber-Producing Grass Taraxacum Kok-saghyz
作者:Xie, Quanliang;Zhu, Liping;Yu, Li;Yuan, Boxuan;Gao, Xuan;Li, Hongbin;Wang, Xuchu;Xie, Quanliang;Ding, Guohua;Zhu, Liping;Yu, Li;Yuan, Boxuan;Liu, Yang;Wang, Xuchu;Wang, Dan;Sun, Yong
关键词:natural rubber biosynthesis; mass spectrometry; rubber grass; rubber latex; shotgun proteomics; Taraxacum kok-saghyz; two-dimensional gel electrophoresis; visual proteome map
-
Comparative Proteomic Analysis of the Hevea brasiliensis Latex under Ethylene and Calcium Stimulation
作者:Wu, Bingsun;Gao, Le;Sun, Yong;Wu, Min;Wei, Jiashao;Wang, Guihua;Wu, Wenguan;He, Peng;Xiao, Junhan;Wang, Xuchu;Wang, Dan;Wang, Xuchu
关键词:Hevea brasiliensis; ethylene; calcium; rubber latex; signal transduction; rubber particle aggregation
-
Comparative physiological and proteomic analyses of the chloroplasts in halophyte Sesuvium portulacastrum under differential salt conditions
作者:Peng, Cunzhi;Chang, Lili;Yang, Qian;Tong, Zheng;Wang, Dan;Tan, Yanhua;Yi, Xiaoping;Wang, Xuchu;Peng, Cunzhi;Chang, Lili;Ding, Guohua;Xiao, Junhan;Zhang, Ying;Wang, Xuchu;Sun, Yong
关键词:Comparative proteomics; Chloroplast; Salt stress; Sesuvium portulacastrum; True halophyte