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Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex

文献类型： 外文期刊

作者： Yu, Li 1 ; Yuan, Boxuan 1 ; Wang, Lingling 2 ; Sun, Yong 3 ; Ding, Guohua 2 ; Souleymane, Ousmane Ahmat 2 ; Zhang, Xue 1 ;

作者机构： 1.Shihezi Univ, Coll Life Sci, Shihezi 832003, Peoples R China

2.Hainan Normal Univ, Coll Life Sci, Key Lab Ecol Trop Isl, Minist Educ, Haikou 571158, Hainan, Peoples R China

3.Chinese Acad Trop Agr Sci, Rubber Res Inst, Danzhou 571737, Peoples R China

关键词： comparative proteomics; Hevea brasiliensis; Hydroxymethylglutaryl-CoA synthase; glycosylated proteins; natural rubber biosynthesis; rubber latex

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 15 期

页码：

收录情况： SCI

摘要： Natural rubber is an important industrial material, which is obtained from the only commercially cultivated rubber tree,Hevea brasiliensis. In rubber latex production, ethylene has been extensively used as a stimulant. Recent research showed that post-translational modifications (PTMs) of latex proteins, such as phosphorylation, glycosylation and ubiquitination, are crucial in natural rubber biosynthesis. In this study, comparative proteomics was performed to identify the glycosylated proteins in rubber latex treated with ethylene for different days. Combined with Pro-Q Glycoprotein gel staining and mass spectrometry techniques, we provided the first visual profiling of glycoproteomics of rubber latex and finally identified 144 glycosylated protein species, including 65 differentially accumulated proteins (DAPs) after treating with ethylene for three and/or five days. Gene Ontology (GO) functional annotation showed that these ethylene-responsive glycoproteins are mainly involved in cell parts, membrane components and metabolism. Pathway analysis demonstrated that these glycosylated rubber latex proteins are mainly involved in carbohydrate metabolism, energy metabolism, degradation function and cellular processes in rubber latex metabolism. Protein-protein interaction analysis revealed that these DAPs are mainly centered on acetyl-CoA acetyltransferase and hydroxymethylglutaryl-CoA synthase (HMGS) in the mevalonate pathway for natural rubber biosynthesis. In our glycoproteomics, three protein isoforms of HMGS2 were identified from rubber latex, and only one HMGS2 isoform was sharply increased in rubber latex by ethylene treatment for five days. Furthermore, theHbHMGS2gene was over-expressed in a model rubber-producing grassTaraxacum Kok-saghyzand rubber content in the roots of transgenic rubber grass was significantly increased over that in the wild type plant, indicating HMGS2 is the key component for natural rubber production.

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