文献类型: 外文期刊
作者: Bai, Changming 1 ; Wang, Chongming 1 ; Xia, Junyang 1 ; Sun, Hailin 3 ; Zhang, Shuai 1 ; Huang, Jie 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao, Peoples R China
2.Qingdao Natl Oceanog Lab, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
3.Changdao Cty Ocean & Fishery Adm, Ya
关键词: Chlamys farreri;Crassostrea gigas;Ostreid herpesvirus 1;OsHV-1;Mass mortality;Molecular epidemiology
期刊名称:JOURNAL OF INVERTEBRATE PATHOLOGY ( 影响因子:2.841; 五年影响因子:3.368 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Viral infection caused by Ostreid herpesvirus 1 (OsHV-1) is one of the proximate causes of mass mortalities of cultivated bivalves around the world. The emergence and spread of different variants of OsHV-1 accompanied by different epidemiological characteristics have been reported frequently in different countries around the world. In this paper, we present a study of the detection of OsHV-1 DNA and their variations from 1599 samples over 18 species collected in 27 aquaculture sites and two food markets during 2001-2013 in China. All of the samples were examined by a nested PCR assay targeting the C2/C6 fragment of OsHV-1 followed by sequencing. Our results showed 338 individuals (21.1%) of seven species sampled from 14 (14/27 = 51.9%) sites and the two food markets were positive for viral DNA. Sequencing of 289 PCR products revealed 24 virus types. No shared virus type was found among different countries with 47 types (23 in Japan, 16 in France, 2 in South Korea and 1 in each country of Australia, USA, Ireland, New Zealand, Mexico and China) identified in previous studies. As previously reported, two main phylogenetic groups were identified by phylogenetic analysis based on the 71 virus types; within which 6 separate clades were identified.
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