您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genome-wide discovery of novel and conserved microRNAs in white shrimp (Litopenaeus vannamei)

文献类型： 外文期刊

作者： Xi, Qian-Yun 1 ; Xiong, Yuan-Yan; Wang, Yuan-Mei 1 ; Cheng, Xiao 1 ; Qi, Qi-En 1 ; Shu, Gang 1 ; Wang, Song-Bo 1 ; Wan 1 ;

作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China

2.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed,

关键词： Genome-wide;MicroRNAs;Solexa deep sequencing;Polysaccharide;White shrimp (Litopenaeus vannamei)

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Of late years, a large amount of conserved and species-specific microRNAs (miRNAs) have been performed on identification from species which are economically important but lack a full genome sequence. In this study, Solexa deep sequencing and cross-species miRNA microarray were used to detect miRNAs in white shrimp. We identified 239 conserved miRNAs, 14 miRNA* sequences and 20 novel miRNAs by bioinformatics analysis from 7,561,406 high-quality reads representing 325,370 distinct sequences. The all 20 novel miRNAs were species-specific in white shrimp and not homologous in other species. Using the conserved miRNAs from the miRBase database as a query set to search for homologs from shrimp expressed sequence tags (ESTs), 32 conserved computationally predicted miRNAs were discovered in shrimp. In addition, using microarray analysis in the shrimp fed with Panax ginseng polysaccharide complex, 151 conserved miRNAs were identified, 18 of which were significant up-expression, while 49 miRNAs were significant down-expression. In particular, qRT-PCR analysis was also performed for nine miRNAs in three shrimp tissues such as muscle, gill and hepatopancreas. Results showed that these miRNAs expression are tissue specific. Combining results of the three methods, we detected 20 novel and 394 conserved miRNAs. Verification with quantitative reverse transcription (qRT-PCR) and Northern blot showed a high confidentiality of data. The study provides the first comprehensive specific miRNA profile of white shrimp, which includes useful information for future investigations into the function of miRNAs in regulation of shrimp development and immunology.

  • 相关文献

[1]Identification and characterization of microRNAs in the zoonotic fluke Fasciolopsis buski. Chen, Mu-Xin,Hu, Wei,Ai, Lin,Chen, Jia-Xu,Chen, Mu-Xin,Hu, Wei,Li, Juan,He, Jun-Jun.

[2]MicroRNAs Involved in Skeletal Muscle Differentiation. Zhang, Xiquan. 2013

[3]A-type ECG and EGCG dimers disturb the structure of 3T3-L1 cell membrane and strongly inhibit its differentiation by targeting peroxisome proliferator-activated receptor gamma with miR-27 involved mechanism. Zhu, Wei,Zou, Bo,Nie, Rongzu,Zhang, Ying,Li, Chun-mei,Zou, Bo,Li, Chun-mei.

[4]Antioxidant Activity of Polysaccharide-enriched Fractions Extracted from Pulp Tissue of Litchi Chinensis Sonn.. Kong, Fanli,Zhang, Mingwei,Liao, Sentai,Chi, Jianwei,Wei, Zhencheng,Kong, Fanli,Yu, Shujuan. 2010

[5]Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp. Yi, Yang,Huang, Fei,Zhang, Ming-Wei,Zhang, Rui-Fen,Deng, Yuan-Yuan,Wei, Zhen-Cheng,Yi, Yang,He, Jing-Ren. 2013

[6]Composition analysis and anti-proliferation activity of polysaccharides from Dendrobium chrysotoxum. Sun, Yan-Dong,Wang, Zai-Hua,Ye, Qing-Sheng,Wang, Zai-Hua.

[7]Effects of alkali dissociation on the molecular conformation and immunomodulatory activity of longan pulp polysaccharide (LPI). Yi, Yang,Zhang, Ming-Wei,Liao, Sen-Tai,Zhang, Rui-Fen,Deng, Yuan-Yuan,Wei, Zhen-Cheng,Yi, Yang,Liao, Sen-Tai,Zhang, Rui-Fen,Deng, Yuan-Yuan,Wei, Zhen-Cheng,Yang, Bao.

[8]Structural features and immunomodulatory activities of polysaccharides of longan pulp. Yi, Yang,Zhang, Ming-Wei,Liao, Sen-Tai,Zhang, Rui-Fen,Deng, Yuan-Yuan,Wei, Zhen-Cheng,Tang, Xiao-Jun,Zhang, Yan,Yi, Yang.

[9]Antioxidant activities of different fractions of polysaccharide purified from pulp tissue of litchi (Litchi chinensis Sonn.). Kong, Fan-Li,Zhang, Ming-Wei,Chi, Jian-Wei,Wei, Zhen-Cheng,Kong, Fan-Li,Yu, Shu-Juan,Kuang, Rui-Bin.

[10]Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia. Deng, Yuan-Yuan,Deng, Yuan-Yuan,Yi, Yang,Zhang, Li-Fang,Zhang, Rui-Fen,Zhang, Yan,Wei, Zhen-Cheng,Tang, Xiao-Jun,Zhang, Ming-Wei,Deng, Yuan-Yuan.

[11]Investigation of the Maillard Reaction between Polysaccharides and Proteins from Longan Pulp and the Improvement in Activities. Han, Miao-Miao,Yi, Yang,Wang, Hong-Xun,Huang, Fei.

[12]Polysaccharides from Laminaria japonica: Structural characteristics and antioxidant activity. Cui, Chun,Lu, Jianghong,Sun-Waterhouse, Dongxiao,Sun, Weizheng,Zhao, Mouming,Zhao, Haifeng,Mu, Lixia,Mu, Lixia,Mu, Lixia.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台