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The vitamin D receptor localization and mRNA expression in ram testis and epididymis

文献类型： 外文期刊

作者： Jin, Hui 1 ; Huang, Yang 1 ; Jin, Guang 2 ; Xue, Yanrong 2 ; Qin, Xiaowei 1 ; Yao, Xiaolei 1 ; Yue, Wenbing 1 ;

作者机构： 1.Shanxi Agr Univ, Coll Anim Sci & Technol, Taigu 030801, Peoples R China

2.Shanxi Acad Agr Sci, Anim Husb & Vet Res Inst, Taiyuan, Peoples R China

关键词： Epididymis;Ram;Testis;VDR

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）

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收录情况： SCI

摘要： The objectives of present study were to investigate the presence of vitamin D receptor (VDR) in testis and epididymis of ram by polymerase chain reaction (PCR), to locate VDR in testis and epididymis by immunohistochemistry and to compare difference of VDR expression between testis and epididymis before and after sexual maturation by Real time-PCR and Western blot. The results showed that VDR exists in the testis and epididymis of ram while VDR protein in testis and epididymis was localized in Leydig cells, spermatogonial stem cells, spermatocytes, Sertoli cells and principal cells. For the adult ram, the amounts of VDR mRNA and VDR protein were less (p < 0.01) in testis than compared with caput, corpus and cauda epididymis. For prepubertal ram, the result showed the same trend (p <0.01). However, the expression levels of VDR mRNA and VDR protein in caput, corpus, cauda epididymis and testis showed no significant difference (p >0.05) between adult and prepubertal. In conclusion, VDR exists in testis and epididymis of ram, suggesting l alpha,25-(OH)(2)VD3 may play a role in ram reproduction. (C) 2014 Elsevier B.V. All rights reserved.

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