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Analysis of Endophytic Bacterial Community Composition by 16S rDNA Clone Library in Achnatherum inebrians

文献类型： 外文期刊

作者： Shi, Ying Wu 1 ; Zhang, Xuebing 1 ; Lou, Kai 1 ;

作者机构： 1.Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi 830091, Peoples R China

关键词： endophytic bacteria;Achnatherum inebriants;bacterial diversity;16S rDNA library

期刊名称：MICROBIOLOGY （ 影响因子：1.156； 五年影响因子：1.308 ）

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收录情况： SCI

摘要： The endophytic bacterial diversity of drunken horse grass (DHG) (Achnatherum inebrians) growing in native habitat station in Xinjiang Province, China was analyzed by 16S rDNA cloning, amplified ribo-somal DNA restriction analysis (ARDRA), and sequence homology comparison. To effectively exclude the interference of chloroplast DNA and mitochondrial DNA of DHG, a pair of bacterial PCR primers was selected to specifically amplify bacterial 16S rDNA sequences directly from DHG root tissues. Among 249 positive clones in the 16S rDNA library of endophytes, 57 OTUs were identified based on the similarity of the ARDRA banding profiles. Sequence analysis revealed diverse phyla of bacteria in the 16S rDNA library, which consisted of alpha, beta, gamma, delta, and epsilon subclasses of the Proteobacteria, Flavobacteria, Actinobacteria, Sphingobacteria, and uncultured bacteria. The dominant group was Proteobacteria, whie the most numerous genus was Bacillus. More than 8.00% of the total clones showed a high similarity to uncultured bacteria, suggesting that nonculturable bacteria make a significant part of DHG endophytic bacterial community. To our knowledge, this is the first report that endophytic bacteria associated with DHG are revealed by the culture-independent approach. The results suggest that the diversity of endophytic bacteria is abundant in DHG.

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[1]Growth promotion effects of the endophyte Acinetobacter johnsonii strain 3-1 on sugar beet. Shi, Yingwu,Lou, Kai,Shi, Yingwu,Li, Chun. 2011

[2]Illumina-based analysis of bacterial diversity related to halophytes Salicornia europaea and Sueada aralocaspica. Sh, Ying-wu,Lou, Kai,Li, Chun,Wang, Lei,Zhao, Zhen-yong,Zhao, Shuai,Tian, Chang-yan. 2015

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