Development of a SCAR (Sequence-characterised amplified region) marker for acid resistance-related gene in Lactobacillus plantarum
文献类型： 外文期刊
作者： Liu, Shu-Wen 1 ; Li, Kai 2 ; Yang, Shi-Ling 1 ; Tian, Shu-Fen 2 ; He, Ling 3 ;
作者机构： 1.Northwest A&F Univ, Coll Enol, Yangling 712100, Shaanxi, Peoples R China
2.Tianjin Acad Agr Sci, Grape Res Ctr, Tianjin 300112, Peoples R China
3.Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China
关键词： Lactobacillus plantarum;Acid-resistant gene;RAPD marker;SCAR marker
期刊名称：EXTREMOPHILES （ 影响因子：2.395； 五年影响因子：2.668 ）
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收录情况： SCI
摘要： A sequence characterised amplified region marker was developed to determine an acid resistance-related gene in Lactobacillus plantarum. A random amplified polymorphic DNA marker named S116-680 was reported to be closely related to the acid resistance of the strains. The DNA band corresponding to this marker was cloned and sequenced with the induction of specific designed PCR primers. The results of PCR test helped to amplify a clear specific band of 680 bp in the tested acid-resistant strains. S116-680 marker would be useful to explore the acid-resistant mechanism of L. plantarum and to screen desirable malolactic fermentation strains.
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