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Determination of 21 Free Amino Acids in Fruit Juices by HPLC Using a Modification of the 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate (AQC) Method

文献类型： 外文期刊

作者： Zeng, Fang; Ou, Jun 1 ; Huang, Yufen 1 ; Li, Qian 1 ; Xu, Guizhi 2 ; Liu, Zhongzhen; Yang, Shaohai;

作者机构： 1.Guangdong Acad Agr Sci, Key Lab Plant Nutr & Fertilizer South Reg, Guangdong Key Lab Nutrient Cycling & Farmland Con, Minist Agr,Inst Agr Resources & Environm, Guangzhou 510640, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Key Lab Plant Nutr & Fertilizer South Reg, Guangdong Key Lab Nutrient Cycling & Farmland Con, Minist Agr,Inst Agr Resources & Environm, Guangzhou 510640, Guangdong, Peoples R C

关键词： Free amino acid;High-performance liquid chromatography;6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate;Fruit juices

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.366； 五年影响因子：3.07 ）

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收录情况： SCI

摘要： The present paper reports the application of an optimized pre-column derivatization procedure with aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) to the determination of 21 free amino acids in fruit juices. The method permitted the optimal separation of 21 amino acids and ammonium in 30 min. Excellent response linearity was obtained from 2 to 1,000 mu M for all amino acids, except tryptophan, for which the linearity was 5-1,000 mu M. The fluorescence detection limits ranged from 0.08 pmol (phenylalanine) to 0.69 pmol (cysteine), except for tryptophan (7.89 pmol), for a signal-to-noise ratio of 3. The relative standard deviations of peak areas ranged from 0.1 to 0.4 % for intraday analysis and from 2.1 to 5.0 % for interday analysis. The method was applied to analyze the free amino acids in six fruit juices, and the recoveries of the proposed method were 90.4 to 103.3 %. The total free amino acid content ranged from 56.97 mg L-1 (apple) to 469.45 mg L-1 (longan) in six fruit juices. Aspartic acid (Asp), glutamic acid (Glu), asparagine (Asn), serine (Ser), and glutamine (Gln) were present in higher levels in most samples, whereas cysteine (Cys) was below the detection limit in the selected samples. Notable advantages of this method include its speed, ease, low cost, good repeatability, high sensitivity, excellent precision and accuracy, and anti-interference in high-sugar matrices.

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