Identification of microRNAs by small RNA deep sequencing for synthetic microRNA mimics to control Spodoptera exigua
文献类型： 外文期刊
作者： Zhang, Yu Liang 1 ; Huang, Qi Xing 1 ; Yin, Guo Hua; Lee, Samantha; Jia, Rui Zong 1 ; Liu, Zhi Xin 1 ; Yu, Nai T 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Minist Agr, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Minist Agr, Key Lab Biol & Genet Resources Trop
关键词： Beet armyworm;Transgenic Bt cotton;Insect resistance;MicroRNAs;Larval development
期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Beet armyworm, Spodoptera exigua, is a major pest of cotton around the world. With the increase of resistance to Bacillus thuringiensis (Bt) toxin in transgenic cotton plants, there is a need to develop an alternative control approach that can be used in combination with Bt transgenic crops as part of resistance management strategies. MicroRNAs (miRNAs), a non-coding small RNA family (18-25 nt), play crucial roles in various biological processes and over-expression of miRNAs has been shown to interfere with the normal development of insects. In this study, we identified 127 conserved miRNAs in S. exigua by using small RNA deep sequencing technology. From this, we tested the effects of 11 miRNAs on larval development. We found three miRNAs, Sex-miR-10-1a, SexmiR-4924, and Sex-miR-9, to be differentially expressed during larval stages of S. exigua. Oral feeding experiments using synthetic miRNA mimics of Sex-miR-1 0-1a, Sex-miR-4924, and S ex-mi R-9 resulted in suppressed growth of S. exigua and mortality. Over-expression of Sex-miR-4924 caused a significant reduction in the expression level of chitinase 1 and caused abortive molting in the insects. Therefore, we demonstrated a novel approach of using miRNA mimics to control S. exigua development. (C) 2014 Elsevier B.V. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
MeGT2.6 increases cellulose synthesis and active gibberellin content to promote cell enlargement in cassava
作者：Bao, Ruxue;Zeng, Changying;Li, Ke;Li, Mengtao;Zhou, Xincheng;Huang, Dongyi;Wang, Wenquan;Chen, Xin;Li, Yajun;Zhou, Xincheng;Wang, Haiyan;Wang, Yajie;Chen, Xin;Li, Yajun;Zhou, Xincheng;Wang, Haiyan;Wang, Yajie;Chen, Xin;Zhou, Xincheng;Wang, Yajie;Chen, Xin
关键词：cassava; gibberellin; cellulose synthase; cell wall; transcription factor
-
Effect of biodegradable PBAT microplastics on the C and N accumulation of functional organic pools in tropical latosol
作者：Chen, Miao;Zhang, Wen;Chen, Xin;Liu, Huiran;Ning, Ziyu;Fan, Changhua;Wu, Dongming;Li, Qinfen;Chen, Miao;Zhang, Wen;Chen, Xin;Ning, Ziyu;Fan, Changhua;Wu, Dongming;Li, Qinfen;Peng, Licheng;Cao, Ming;Zhang, Meng;Wu, Dongming
关键词：Particulate organic matter; Mineral-associated organic matter; Carbon and nitrogen cycling; Microbial community; Enzyme activity
-
Physicochemical properties, multi-elemental composition, and antioxidant activity of five unifloral honeys from Apis cerana cerana
作者：Wu, Jiao;Zhao, Shan;Chen, Xin;Liu, Junfeng;Gao, Jinglin;Wang, Shijie;Jiu, Yuanda;Liu, Junfeng
关键词：Unifloral honey; Physicochemical properties; Element analysis; Antioxidant activity
-
Organic substitution stimulates ammonia oxidation-driven N2O emissions by distinctively enriching keystone species of ammonia-oxidizing archaea and bacteria in tropical arable soils
作者：Zhang, Qi;Wang, Xiaotong;Fu, Yajun;Zhao, Xiongwei;Zhang, Qi;Chen, Miao;Leng, Youfeng;Wang, Xiaotong;Fu, Yajun;Wang, Danfeng;Zhao, Xiongwei;Gao, Wenlong;Li, Ning;Chen, Xin;Fan, Changhua;Li, Qinfen;Chen, Miao;Gao, Wenlong;Li, Ning;Chen, Xin;Fan, Changhua;Li, Qinfen;Chen, Miao;Gao, Wenlong;Li, Ning;Chen, Xin;Fan, Changhua;Li, Qinfen;Leng, Youfeng;Wang, Danfeng;Fan, Changhua;Li, Qinfen
关键词：Ammonia oxidation; Partial organic substitution; Nitrous oxide; Community structure; Tropical arable soil
-
Factors predictive of the biogeographic distribution of comammox Nitrospira in terrestrial ecosystems
作者：Gao, Wenlong;Fan, Changhua;Zhang, Wen;Li, Ning;Liu, Huiran;Chen, Xin;Liu, Yuqin;Wu, Xiaolong;Li, Qinfen;Chen, Miao;Gao, Wenlong;Fan, Changhua;Zhang, Wen;Li, Ning;Liu, Huiran;Chen, Xin;Liu, Yuqin;Wu, Xiaolong;Li, Qinfen;Chen, Miao;Gao, Wenlong;Fan, Changhua;Zhang, Wen;Li, Ning;Liu, Huiran;Chen, Xin;Liu, Yuqin;Wu, Xiaolong;Li, Qinfen;Chen, Miao;Gao, Wenlong;Fan, Changhua;Zhang, Wen;Li, Ning;Liu, Huiran;Chen, Xin;Liu, Yuqin;Wu, Xiaolong;Li, Qinfen;Chen, Miao;Gao, Wenlong;Fan, Changhua;Zhang, Wen;Li, Ning;Liu, Huiran;Chen, Xin;Liu, Yuqin;Wu, Xiaolong;Li, Qinfen;Chen, Miao;Fu, Yajun;Wang, Yongsheng
关键词：Comammox Nitrospira; Ecological preference; Predictive factors; Incomplete nitrifiers; Population co-variation; Random forest modelling
-
Heterotrophic ammonium oxidation is not active in acidic paddy soils
作者：Gao, Wenlong;Fan, Changhua;Zhang, Wen;Li, Ning;Liu, Huiran;Chen, Xin;Liu, Yuqin;Wu, Xiaolong;Chen, Miao;Gao, Wenlong;Fan, Changhua;Zhang, Wen;Li, Ning;Liu, Huiran;Chen, Xin;Liu, Yuqin;Wu, Xiaolong;Chen, Miao;Gao, Wenlong;Fan, Changhua;Zhang, Wen;Li, Ning;Liu, Huiran;Chen, Xin;Liu, Yuqin;Wu, Xiaolong;Chen, Miao;Gao, Wenlong;Fan, Changhua;Zhang, Wen;Li, Ning;Liu, Huiran;Chen, Xin;Liu, Yuqin;Wu, Xiaolong;Chen, Miao;Gao, Wenlong;Fan, Changhua;Zhang, Wen;Liu, Huiran;Chen, Xin;Liu, Yuqin;Wu, Xiaolong;Chen, Miao;Gao, Wenlong;Gao, Wenlong;Zhang, Jinbo;Mueller, Christoph;Mueller, Christoph;Mueller, Christoph;Mueller, Christoph
关键词：Heterotrophic ammonia oxidation; Nitrification inhibitor; N-15-tracing techniques; Transcriptional activity; Acidic paddy soils
-
Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava
作者：Tuo, Decai;Yao, Yuan;Yan, Pu;Chen, Xin;Kong, Hua;Guo, Jianchun;Shen, Wentao;Tuo, Decai;Yao, Yuan;Yan, Pu;Chen, Xin;Kong, Hua;Guo, Jianchun;Shen, Wentao;Qu, Feihong;Xue, Weiqian;Liu, Jinping;Cui, Hongguang;Dai, Zhaoji;Qu, Feihong;Xue, Weiqian;Liu, Jinping;Cui, Hongguang;Dai, Zhaoji
关键词：Cassava; Cassava common mosaic virus; Protein overexpression; Genome editing
