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A method to label biological molecules with dsDNA coated gold nanoparticles

文献类型： 外文期刊

作者： Xing, Ming 1 ; Li, Fangliang 2 ; Dong, Yingshan 1 ; Yu, Zhenxiang 4 ; Chen, Xia 2 ;

作者机构： 1.Jilin Acad Agr Sci, Gongzhuling, Peoples R China

2.Jilin Univ, Natl & Local United Engn Lab Chinese Herbal Med B, Changchun 130023, Peoples R China

3.Jilin Univ, Sch Life Sci, Changchun 130023, Peoples R China

4.Jilin Univ, Hosp 1, Changchun 130023, Peoples R China

关键词： Antibody;Biotin;Double strand DNA;Gold nanoparticle;In situ hybridization;Streptavidin

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

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收录情况： SCI

摘要： We described a new method to label biological molecules using gold nanoparticles (GNPs) and double stranded DNA. Researchers can conveniently label their own samples with GNPs using this method. The label is based on dsDNA with a 93.5 % coverage of GNPs (dsDNA:GNP = 303:1). Antigens, streptavidin and biotin were labeled on GNPs and the success of the method was investigated with agarose gel electrophoresis, laser particle size analysis and ultraviolet spectrophotometry. These analyses confirmed that biological molecules were successfully bound to the GNPs. These molecules retained their biological activity and were able to detect targets on PVDF and NC membranes with excellent selectivity and low levels of background. Modified GNPs were also able to detect targets on nylon membranes, but with some degree of false positives. The maximum limit of detection was 25 ng proteins.

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