Effects of glycerol on pigments and monacolin K production by the high-monacolin K-producing but citrinin-free strain, Monascus pilosus MS-1
文献类型: 外文期刊
作者: Feng, Yanli 1 ; Shao, Yanchun 1 ; Zhou, Youxiang 4 ; Chen, Fusheng 1 ;
作者机构: 1.Huazhong Agr Univ, Key Lab Environm Correlat Dietol, Minist Educ, Wuhan 430070, Hubei Province, Peoples R China
2.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei Province, Peoples R China
3.Hubei Normal Univ, Coll Life Sci, Huangshi 435000, Hubei Province, Peoples R China
4.Hubei Acad Agr Sci, Inst Qual Stand & Testing Technol AgroProd, Wuhan 430070, Hubei Province, Peoples R China
关键词: Monascus pilosus MS-1;Glycerol;Pigments;Monacolin K;Citrinin
期刊名称:EUROPEAN FOOD RESEARCH AND TECHNOLOGY ( 影响因子:2.998; 五年影响因子:3.005 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Glycerol is the principal by-product of bio-diesel production which can be utilized by fungi for beneficial metabolites production. In this study, effects of glycerol on main metabolites production and gene expression of pigment and monacolin K (MK) synthesis-related genes of Monascus pilosus MS-1, a high-MK-producing but citrinin-free strain, were investigated. The results indicated that addition of glycerol could increase the dry cell weight but decrease the fermentation broth pH value. Pigments production could be enhanced significantly after adding 2 g/L glycerol and the maximum pigments yield (13.78 U/mL) of fermentation broth was obtained when the glycerol content was up to 40 g/L (p < 0.05). Effect of glycerol on MK production was different from that of Monascus pigment. MK yield of fermentation broth was decreased significantly when the glycerol addition was up to 10 g/L while that of mycelia could be improved as glycerol addition increased (p < 0.05). Results of real-time PCR revealed that when the glycerol additions were up to 40 and 300 g/L, respectively, the gene expressions of regulatory gene (ctnR) for pigment synthesis were 17.31- and 22.22-folds of ctnR in the control; the gene expressions of structural gene (PKS5) for pigment synthesis were 33.27- and 60.07-folds of PKS5 in the control; respectively. However, the gene expressions of regulatory (mkH) and structural (mkA) genes of MS-1 for MK production were decreased after adding glycerol. All this suggesting that glycerol can be used for improving production of citrinin-free Monascus pigments and MK.
- 相关文献
作者其他论文 更多>>
-
One-Pot Preparation of Mixed-Mode Reversed-Phase Anion-Exchange Silica Sorbent and its Application in the Detection of Cyclopiazonic Acid in Feeds and Agricultural Products
作者:Hu, Xuan;Liu, Li;Peng, Maomin;Zheng, Dan;Xia, Hong;Zhou, Youxiang;Peng, Lijun;Hu, Xuan;Liu, Li;Peng, Maomin;Zheng, Dan;Xia, Hong;Zhou, Youxiang;Peng, Xitian
关键词:cyclopiazonic acid; pyridine and octyl co-bonded silica; mixed-mode; agricultural products; UPLC-MS/MS
-
mrvam 7, a conserved SNARE gene involved in vacuolar fusion, is required for development and secondary metabolism in Monascus ruber M7
作者:Meng, Chenchen;Chen, Fusheng;Zhou, Youxiang;Liu, Jiao;Gao, Zhipeng
关键词:Monascus ruber; mrvam 7; SNAREs; Vacuolar fusion; Development; Secondary metabolism
-
Fluorescence Quenching Detection of Clothianidin in Fruit and Vegetable Samples Using MAPbBr3 Perovskite Quantum Dots
作者:Liu, Li;Peng, Maomin;Xia, Hong;Peng, Xitian;Peng, Lijun;Zhou, Youxiang;Liu, Li;Peng, Maomin;Xia, Hong;Peng, Xitian;Peng, Lijun;Zhou, Youxiang;Liu, Li;Zhang, Jin Z.;Xu, Ke
关键词:perovskite; quantum dots; clothianidin; fluorescence detection; neonicotinoids
-
The impacts of animal agriculture on One Health-Bacterial zoonosis, antimicrobial resistance, and beyond
作者:Zhang, Tengfei;Zhang, Wenting;Shang, Yu;Luo, Qingping;Wen, Guoyuan;Nickerson, Rhea;Cheng, Zhenyu;Peng, Xitian;Zhou, Youxiang;Peng, Xitian;Zhou, Youxiang;Peng, Xitian;Zhou, Youxiang;Luo, Qingping
关键词:Zoonosis; Antimicrobial resistance
-
Exploring the Subcellular Localization of Monascus Pigments Biosynthases: Preliminary Unraveling of the Compartmentalization Mechanism
作者:Xiong, Fei;Wei, Jingyi;Shao, Yanchun;Chen, Fusheng;Zhou, Youxiang;Liu, Jiao
关键词:Monascus spp.; Monascus pigments; Monascus pigments biosynthetic proteins; subcellular localization; compartmentalization
-
Histone lysine methyltransferases MpDot1 and MpSet9 are involved in the production of lovastatin and MonAzPs by histone crosstalk modification
作者:Gong, Yunxia;Li, Shengfa;Chen, Fusheng;Shao, Yanchun;Zhou, Youxiang;Chen, Fusheng;Shao, Yanchun
关键词:Histone methylation; Histone crosstalk; Transcriptional regulation; Lovastatin; Monascus -type azaphilone pigments
-
Comparative Analysis of the Physicochemical Properties and Metabolites of Farinose and Crisp Lotus Roots (Nelumbo nucifera Gaertn.) with Different Geographical Origins
作者:Liu, Jiao;Yang, Jie;Wang, Jing;Peng, Xitian;Yan, Wei;Peng, Lijun;Zhou, Youxiang;Peng, Jiawen;Zhao, Liuqing
关键词:lotus root; texture; metabolites; Zeno trap; multivariate analysis