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Molecular polymorphism of chlorpyrifos-degrading strains in soil treated with controlled and non-controlled release formulations of chlorpyrifos

文献类型: 外文期刊

作者: Chen, Liezhong 1 ; Li, Yanli 1 ; Wang, Ting 1 ; Yu, Yunlong 2 ;

作者机构: 1.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310029, Zhejiang, Peoples R China

关键词: Chlorpyrifos;Soil;Degrading microorganism;Molecular polymorphism;Microcapsules;Emulsifiable concentrate

期刊名称:APPLIED SOIL ECOLOGY ( 影响因子:4.046; 五年影响因子:4.884 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The molecular polymorphisms of chlorpyrifos (CPF)-degrading strains in soil treated with microcapsule (MC) and emulsifiable concentrate (EC) formulations of CPF were studied by laboratory experiments. Results showed that CPF residue in MC-treated soil was maintained at a certain level during 60 days, and the residual period reached 120 days. CPF residue decreased via a first-order model with a residual period of 60 days in EC-treated soil. A total of 16 and 9 degrading strains capable of utilizing CPF as a sole source of carbon and energy were isolated from these two treatments, respectively. All degrading strains were grouped into 16 different types based on enterobacterial repetitive intergenic consensus (ERIC)-PCR pattern similarities. Strains isolated from MC- and EC-treated soils belonged to 14 and 8 ERIC-types, respectively. Six groups of strains that were isolated from MC-and EC-treated soils belonged to similar ERIC-types. All ERIC-type strains were similar to nine known species according to BLAST analysis at the NCBI website. The results indicated that the population and degradation abilities of CPF-degrading strains in soil were visibly strengthened by the controlled-release formulation of CPF compared with those by the non-controlled release formulation. (C) 2014 Elsevier B.V. All rights reserved.

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