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Protein sHSP26 improves chloroplast performance under heat stress by interacting with specific chloroplast proteins in maize (Zea mays)

文献类型： 外文期刊

作者： Hu, Xiuli 1 ; Yang, Yanfang 1 ; Gong, Fangping 1 ; Zhang, Dayong 2 ; Zhang, Li 1 ; Wu, Liuji 1 ; Li, Chaohao 1 ; Wang, We 1 ;

作者机构： 1.Henan Agr Univ, Coll Agron, Coll Life Sci, State Key Lab Wheat & Maize Crop Sci, Zhengzhou 450002, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Biotechnol, Prov Key Lab Agrobiol, Nanjing 210094, Jiangsu, Peoples R China

关键词： sHSP26;Chloroplast proteins;Heat stress;Heat shock protein;Maize protoplast;Protein interaction

期刊名称：JOURNAL OF PROTEOMICS （ 2020影响因子：4.044； 五年影响因子：4.02 ）

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收录情况： SCI

摘要： We recently demonstrated that chloroplast small HSP26 (sHSP26) is abundant in maize leaves under heat stress and potentially involved in maize heat tolerance. However, it largely remains unclear how sHSP26 functions in maize under heat stress. Here, 2-DE-based proteomics, RNA interference (RNAi), co-immunoprecipitation (Co-IP) and yeast two-hybrid (Y2H) were used to reveal chloroplast proteins interacting with sHSP26 and how sHSP26 functions under heat stress. After the silencing of sHSP26, a total of 45 protein spots from isolated protoplasts were greatly changed in abundance, of which 33 spots are chloroplastic. Co-IP revealed that nine proteins possibly associated with 5HSP26. Y2H demonstrated that six chloroplast proteins interact with 5HSP26 under heat stress. In particular, four proteins, including ATP synthase subunit beta, chlorophyll a b binding protein, oxygen-evolving enhancer protein 1 and photosystem I reaction center subunit IV, strongly interacted with sHSP26 and their abundance greatly declined after RNAi of 5HSP26 under heat stress. In addition, H2O2 accumulation in the chloroplasts significantly increased the expression of sHSP26, and the suppression of sHSP26 expression significantly reduced the O-2 evolution rate of photosystem II under heat stress. Overall, these findings demonstrate the relevance of sHSP26 in protecting maize chloroplasts under heat stress.

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