Changes in Oleic Acid Content of Transgenic Soybeans by Antisense RNA Mediated Posttranscriptional Gene Silencing
文献类型： 外文期刊
作者： Zhang, Ling 1 ; Yang, Xiang-dong 1 ; Zhang, Yuan-yu 1 ; Yang, Jing 1 ; Qi, Guang-xun 1 ; Guo, Dong-quan 1 ; Xing, Guo- 1 ;
作者机构： 1.Jilin Acad Agr Sci, Agrobiotechnol Res Inst, Changchun 130033, Jilin, Peoples R China
2.Jilin Acad Agr Sci, Agrobiotechnol Res Inst, Changchun 130033, Jili
期刊名称：INTERNATIONAL JOURNAL OF GENOMICS （ 影响因子：2.326； 五年影响因子：2.557 ）
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收录情况： SCI
摘要： The Delta-12 oleate desaturase gene (FAD2-1), which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of seed oil. In this study, we inhibited the expression of endogenous Delta-12 oleate desaturase GmFad2-1b gene by using antisense RNA in soybean Williams 82. By employing the soybean cotyledonary-node method, a part of the cDNA of soybean GmFad2-1b 801 bp was cloned for the construction of a pCAMBIA3300 vector under the soybean seed promoter BCSP. Leaf painting, LibertyLink strip, PCR, Southern blot, qRT-PCR, and fatty acid analysis were used to detect the insertion and expression of GmFad2-1b in the transgenic soybean lines. The results indicate that the metabolically engineered plants exhibited a significant increase in oleic acid (up to 51.71%) and a reduction in palmitic acid (to <3%) in their seed oil content. No structural differences were observed between the fatty acids of the transgenic and the nontransgenic oil extracts.
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