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Actinobacillus pleuropneumoniae two-component system QseB/QseC regulates the transcription of PilM, an important determinant of bacterial adherence and virulence

文献类型： 外文期刊

作者： Liu, Jinlin 1 ; Hu, Linlin 1 ; Xu, Zhuofei 1 ; Tan, Chen 1 ; Yuan, Fangyan 3 ; Fu, Shulin 1 ; Cheng, Hui 1 ; Chen, Huanch 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China

2.Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hubei, Peoples R China

3.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan 430064, Peoples R China

关键词： Actinobacillus pleuropneumoniae;QseB/QseC;Transcriptional regulation;PilM;Adherence;Virulence

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

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收录情况： SCI

摘要： QseB/QseC is one of the five predicted two-component systems (TCSs) in Actinobacillus pleuropneumoniae. To understand the roles of this TCS in A. pleuropneumoniae, a markerless gene-deletion mutant Delta qseBC was constructed. Differentially expressed (DE) genes in Delta qseBC were filtered by microarray analysis. A total of 44 DE genes were found to be regulated by QseB/QseC system. The transcriptional profile of A. pleuropneumoniae Delta qseBC was compared with that of Delta luxS and catecholamine (CA) stimulations, 13 genes regulated by QseB/QseC were found also regulated by LuxS, and 3 Qse-regulons were co-regulated by CA stimulations, respectively. Binding of QseB to the promoters of three regulons (pilM, glpK and hugZ), which were co-regulated by QseB/QseC and LuxS, was evaluated by electrophoretic mobility-shift assay. Results indicated that pilM was directly regulated by phosphorylated-QseB. Then the pilM deletion mutant Delta pilM was constructed and characterized. Data presented here revealed that adherence ability of Delta pilM to St. Jude porcine lung cells was significantly decreased, and Delta pilM exhibited reduced virulence in pigs, suggesting PilM contributes to the process of A. pleuropneumoniae infection. (C) 2015 Elsevier B.V. All rights reserved.

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