您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

An aptamer-based signal-on bio-assay for sensitive and selective detection of Kanamycin A by using gold nanoparticles

文献类型： 外文期刊

作者： Chen, Jing 1 ; Li, Zhaohui 1 ; Ge, Jia 1 ; Yang, Ran 1 ; Zhang, Lin 1 ; Qu, Ling-bo 1 ; Wang, Hong-qi 3 ; Zhang, Ling 3 ;

作者机构： 1.Zhengzhou Univ, Coll Chem & Mol Engn, Zhengzhou 450001, Peoples R China

2.Henan Univ Technol, Sch Chem & Chem Engn, Zhengzhou 450001, Peoples R China

3.Henan Acad Agr Sci, Inst Qual Standard & Testing Technol Agroprod, Zhengzhou 450002, Peoples R China

关键词： Aptamer;Fluorescence;Gold nanoparticles;Kanamycin A

期刊名称：TALANTA （ 影响因子：6.057； 五年影响因子：5.386 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study, a simple and sensitive aptamer-based fluorescence method for the detection of Kanamycin A by using gold nanoparticles (AuNPs) has been developed. In this assay, AuNPs were utilized as DNA nanocarrier as well as efficient fluorescence quencher. In the absence of Kanamycin A, dye-labeled aptamer could be adsorbed onto the surface of AuNPs and the fluorescence signal was quenched. In the presence of Kanamycin A, the specific binding between dye-labeled aptamer and its target induced the formation of rigid structure, which led to dye-labeled aptamer releasing from the surface of AuNPs and the fluorescence intensity was recovered consequently. Under optimum conditions, calibration modeling showed that the analytical linear range covered from 0.8 nM to 350 nM and the detection limit of 0.3 nM was realized successfully. This proposed bio-assay also showed high selectivity over other antibiotics. Meanwhile, this strategy was further used to determine the concentrations of Kanamycin A in milk sample with satisfying results. (C) 2015 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Label-free and rapid detection of ATP based on structure switching of aptamers. Ji, Danyang,Ge, Jia,Zhang, Lin,Li, Jianjun,Bai, Dongmei,Chen, Juan,Li, Zhaohui,Wang, Hongqi,Li, Zhaohui.

[2]A SERS-based multiple immuno-nanoprobe for ultrasensitive detection of neomycin and quinolone antibiotics via a lateral flow assay. Shi, Qiaoqiao,Zhang, Gaiping,Shi, Qiaoqiao,Sun, Yaning,Deng, Ruiguang,Teng, Man,Li, Qingmei,Yang, Yanyan,Hu, Xiaofei,Zhang, Gaiping,Shi, Qiaoqiao,Huang, Jie,Zhang, Zhijun,Zhang, Gaiping. 2018

[3]Nanoparticle orientationally displayed antigen epitopes improve neutralizing antibody level in a model of porcine circovirus type 2. Ding, Peiyang,Li, Yafei,Zhou, Enmin,Zhang, Gaiping,Ding, Peiyang,Zhang, Teng,Li, Yafei,Teng, Man,Sun, Yaning,Liu, Xiao,Chai, Shujun,Jin, Qianyue,Zhang, Gaiping,Zhang, Teng,Liu, Xiao,Zhang, Gaiping,Jin, Qianyue,Zhang, Gaiping. 2017

[4]Highly sensitive and fast responsive ratiometric fluorescent probe for Cu2+ based on a naphthalimide-rhodamine dyad and its application in iving cell imaging. Tang, Jun,Ma, Saige,Liu, Yaqi,Zhao, Yufen,Ye, Yong,Zhang, Di,Zhao, Yufen.

[5]Two rhodamine based chemosensors for Sn4+ and the application in living cells. Cheng, Junye,Yang, Erbing,Ding, Peigang,Tang, Jun,Zhang, Di,Zhao, Yufen,Ye, Yong,Zhang, Di,Zhao, Yufen,Ye, Yong.

[6]Ultrasensitive fluorometric glutathione assay based on a conformational switch of a G-quadruplex mediated by silver(I). Ji, Danyang,Meng, Hongmin,Ge, Jia,Zhang, Lin,Bai, Dongmei,Li, Jianjun,Qu, Lingbo,Li, Zhaohui,Wang, Hongqi,Li, Zhaohui.

[7]An ESIPT-Based Fluorescent Probe for Hydrazine Detection in Aqueous Solution and its Application in Living Cells. Ju, Zhiyu,Li, Dapeng,Li, Dandan,Wu, Changzeng,Xu, Zhihong,Zhang, Di. 2017

[8]A Turn on ESIPT Probe for Rapid and Ratiometric Fluorogenic Detection of Hg2+ and its Application in Live-Cell Imaging. Zhang, Di,Liu, Jihong,Yin, Haiyan,Wang, Hongqi,Li, Shufang,Wang, Min,Li, Man,Zhou, Ling,Zhang, Junfeng,Zhang, Di,Liu, Jihong,Yin, Haiyan,Wang, Hongqi,Li, Shufang,Wang, Min,Li, Man,Zhou, Ling,Zhang, Junfeng,Zhang, Di,Liu, Jihong,Yin, Haiyan,Wang, Hongqi,Li, Shufang,Wang, Min,Li, Man,Zhou, Ling,Zhang, Junfeng. 2016

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台