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Authentication and traceability of fish maw products from the market using DNA sequencing

文献类型： 外文期刊

作者： Wen, Jing 1 ; Zeng, Ling 1 ; Sun, Yulin 1 ; Chen, Daohai 1 ; Xu, Youhou 2 ; Luo, Peng 3 ; Zhao, Zhe 3 ; Yu, Zonghe 3 ; Fan, 1 ;

作者机构： 1.Lingnan Normal Univ, Dept Biol, Zhanjiang 524048, Peoples R China

2.Qinzhou Univ, Guangxi Key Lab Beibu Gulf Marine Biodivers Conse, Qinzhou 535000, Peoples R China

3.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou 510301, Guangdong, Peoples R China

4.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr, Key Lab South China Sea Fishery Resources Utiliza, Guangzhou 510300, Guangdong, Peoples R China

关键词： Fish maw;Genetic identification;Labeling;Traceability;16S rRNA gene;BLAST

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN：

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收录情况： SCI

摘要： Fish maws (dried swimbladders of fishes) are regarded as traditional luxurious delicacies, medicine and tonics, which have been recommended and consumed in Asia over many centuries. At the commercial level, they are ranked as different values based on species. However, fish maw species and the trade are still unknown and undocumented. The processing treatments make them difficult for species identification based on morphological characterization. In the present study, the genetic identification of the main commercialized species of fish maws has been carried out, based on the amplification of a fragment of mitochondrial 16S rRNA gene and subsequent BLAST analysis. The applicability of all kinds of processed. products was verified, including dried, water soaked; salt fried and salt fried plus water soaked forms. The result indicated this method was applicable to all of them, showed that 53.3% of the products were incorrectly labeled and 58.3% of "croaker" products were substituted with catfish or perch species. Moreover, results indicated that besides traditional trade in Asia, the fish maw trade has been globally expanded to meet the growing demand of market. Therefore, this method can be useful in normative control of processed products, particularly in the authenticity of imported species, verifying the correct traceability in commercial trade, the correct labeling, and also for fisheries control of endangered species to conserve stocks biodiversity. (C) 2015 Elsevier Ltd. All rights' reserved.

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