The role of Nrf2 signaling pathway in the mud crab (Scylla paramamosain) in response to Vibrio parahaemolyticus infection
文献类型： 外文期刊
作者： Cheng, ChangHong 1 ; Ma, HongLing 1 ; Liu, GuangXin 1 ; Fan, SiGang 1 ; Deng, YiQin 1 ; Jiang, JianJun 1 ; Feng, Juan 1 ; Guo, ZhiXun 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Guangzhou 510300, Guangdong, Peoples R China
2.Chinese Acad Fishery Sci, Shenzhen Base South China Sea Fisheries Res Inst, Shenzhen, Peoples R China
关键词： Mud crab; Nrf2; Immune response; Bacterial challenge
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）
ISSN： 1050-4648
年卷期： 2023 年 136 卷
页码：
收录情况： SCI
摘要： The transcription factor Nrf2 plays vital roles in detoxification and antioxidant enzymes against oxidative stress. However, the function of Nrf2 in crustaceans is not well studied. In this study, a novel Nrf2 gene from the mud crab (Sp-Nrf2) was identified. It was encoded 245 amino acids. Sp-Nrf2 expression was ubiquitously expressed in all tested tissues, with the highest expression level in the gill. Sp-Nrf2 protein was mainly located in the nucleus. The expression levels of Sp-Nrf2, and antioxidant-related genes (HO-1 and NQO-1) were induced after Vibrio parahaemolyticus infection, indicating that Nrf2 signaling pathway was involved in the responses to bacterial infection. Over-expression of Sp-Nrf2 could improve cell viability after H2O2 exposure, indicating that Sp-Nrf2 might relieve oxidative stress. Silencing of Sp-Nrf2 in vivo decreased HO-1 and NQO-1 expression. Moreover, knocking down Sp-Nrf2 in vivo can increase malondialdehyde content and the mortality of mud crabs after V. parahaemolyticus infection. Our results indicated that Nrf2 signaling pathway played a significant role in immune response against bacterial infection.
- 相关文献
作者其他论文 更多>>
-
The complete mitochondrial genome of bamboo shrimp Atyopsis moluccensis (Atyidae, Decapoda)
作者：Peng, Chao;Fan, Sigang;Fan, Sigang
关键词：Mitochondrial genome; phylogeny; Atyopsis moluccensis; Atyidae
-
Oral delivery of bacteria expressing wsv108 gene-specific dsRNA protects shrimp from white spot syndrome virus (WSSV) infection
作者：Yang, Linwei;Huang, Siyou;Wang, Zi-ang;Han, Deyu;Gan, Yushi;Geng, Ran;Zuo, Hongliang;Weng, Shaoping;He, Jianguo;Xu, Xiaopeng;Yang, Linwei;Huang, Siyou;Wang, Zi-ang;Han, Deyu;Gan, Yushi;Geng, Ran;Zuo, Hongliang;Weng, Shaoping;He, Jianguo;Xu, Xiaopeng;Zuo, Hongliang;Weng, Shaoping;He, Jianguo;Xu, Xiaopeng;Guo, Zhixun;Xu, Xiaopeng
关键词：dsRNA; Oral delivery; White spot syndrome virus
-
Identification and functional characterization of laminin receptor in the mud crab, Scylla paramamosain, in response to MCDV-1 challenge
作者：Zhu, Ke;Cheng, Chang-Hong;Ma, Hong -Ling;Liu, Guang-Xin;Fan, Si-Gang;Deng, Yi-Qin;Jiang, Jian-Jun;Feng, Juan;Guo, Zhi-Xun;Zhu, Ke
关键词：Scylla paramamosain; Laminin receptor; MCDV-1; RNA interference
-
A first report of Streptococcus iniae infection of the spotted sea bass (Lateolabrax maculates)
作者：Deng, Yiqin;Lin, Ziyang;Xu, Liwen;Jiang, Jianjun;Cheng, Changhong;Ma, Hongling;Feng, Juan
关键词：Streptococcus iniae; spotted sea bass (Lateolabrax maculates); LD50; pathogenicity; antibiotic resistance
-
HIF-1-mediated regulation of LDH gene unravels key insights into MCDV-1 pathogenesis in mud crabs Scylla paramamosain
作者：Jie, Yu-Kun;Xie, Jia-Wei;Cheng, Chang-Hong;Jiang, Jian-Jun;Ma, Hong-Ling;Liu, Guang-Xin;Fan, Si-Gang;Deng, Yi-Qin;Feng, Juan;Guo, Zhi-Xun;Jie, Yu-Kun;Xie, Jia-Wei
关键词：LDH; HIF-1; MCDV-1; Scylla paramamosain; Aerobic glycolysis
-
Transcriptome profiling of the gills to air exposure in mud crab Scylla paramamosain
作者：Fan, Sigang;Guo, Yihui;Cheng, Changhong;Huang, Xiaolin;Ma, Hongling;Guo, Zhixun;Liu, Guangxin;Gao, Yougen;Fan, Sigang;Huang, Xiaolin;Yang, Qibin;Fan, Sigang;Yang, Qibin;Gao, Yougen
关键词：Scylla paramamosain; Transcriptome; gill; air exposure
-
Identification and functional characterization of activating transcription factor 6 (ATF6) from the mud crab (Scylla paramamosain) in response to hydrogen peroxide and bacterial challenge
作者：Liu, Guang-Xin;Tian, Yu;Cheng, Chang-Hong;Ma, Hong-Ling;Fan, Si-Gang;Deng, Yi-Qin;Feng, Juan;Jiang, Jian-Jun;Guo, Zhi-Xun
关键词：ATF6; Endoplasmic reticulum stress; Innate immunity; Unfolded protein response
