Oral delivery of bacteria expressing wsv108 gene-specific dsRNA protects shrimp from white spot syndrome virus (WSSV) infection
文献类型： 外文期刊
作者： Yang, Linwei 1 ; Huang, Siyou 1 ; Wang, Zi-ang 1 ; Han, Deyu 1 ; Gan, Yushi 1 ; Geng, Ran 1 ; Zuo, Hongliang 1 ; Guo, Zhixun 4 ; Weng, Shaoping 1 ; He, Jianguo 1 ; Xu, Xiaopeng 1 ;
作者机构： 1.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou, Peoples R China
2.Southern Marine Sci & Engn Guangdong Lab, Zhuhai, Peoples R China
3.Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou, Peoples R China
4.CAFS, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
5.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Peoples R China
关键词： dsRNA; Oral delivery; White spot syndrome virus
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）
ISSN： 0141-8130
年卷期： 2024 年 261 卷
页码：
收录情况： SCI
摘要： Double-stranded RNA (dsRNA) can specifically inhibit gene expression by RNA interference and has important application potential in animal disease control. White spot syndrome virus (WSSV) is one of the most harmful pathogens in shrimp aquaculture, causing huge economic losses every year. In this study, we investigated the function of the WSSV-encoded wsv108 protein. We demonstrated that wsv108 could promote apoptosis by interacting with heat shock protein 70 (HSP70) and enhancing the expression of multiple apoptosis-related genes. Silencing of wsv108 gene by injection with specific dsRNA prepared by in vitro transcription significantly increased the survival rate of WSSV-infected shrimp and reduced the viral load in tissues, suggesting that wsv108 is important for WSSV pathogenicity. Based on this, we expressed the wsv108 specific dsRNA in engineered Escherichia coli. Oral feeding of this bacterium could inhibit the expression of wsv108, increase the survival rate of WSSV-infected shrimp, and decrease the viral load of WSSV in tissues. Therefore, this study developed a new method for treatment of WSSV disease by oral administration of bacterially expressed dsRNA against a novel therapeutic target molecule, which could be a potential candidate strategy for WSSV control in aquaculture.
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