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Effect of NaCl Treatments on Tyramine Biosynthesis of Enterococcus faecalis

文献类型: 外文期刊

作者: Liu, Fang 1 ; Wang, Xinxin 1 ; Du, Lihui 3 ; Wang, Daoying 1 ; Zhu, Yongzhi 1 ; Geng, Zhiming 1 ; Xu, Xiaoxi 2 ; Xu, Weim 1 ;

作者机构: 1.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing 210014, Peoples R China

2.Northeast Agr Univ, Coll Food Sci, Harbin 150030, Peoples R China

3.Nanjing Univ Finance & Econ, Coll Food Sci & Engn, Nanjing 210023, Jiangsu, Peoples R China

期刊名称:JOURNAL OF FOOD PROTECTION ( 影响因子:2.077; 五年影响因子:2.224 )

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收录情况: SCI

摘要: The effect of NaCl stress (0 to 8%, wt/vol) on the growth and tyramine production in two Enterococcus faecalis strains was examined during culture time. The growth of E.faecalis was inhibited by the increase in NaCl concentration, but tyramine production was unaffected. Tyramine accumulated rapidly during the logarithmic phase of the strains, and the final tyramine levels were approximately 800 mu g/ml. Relative gene expression of four genes in the tyrosine decarboxylase locus, namely, tyrRS, tyrDC, tyrP, and nhaC, was evaluated at different incubation times. The results showed that NaCl stress could upregulate the expression of tyrDC and tyrP to improve the tyramine production of a single E. faecalis strain under certain conditions, and TyrS could act as a negative regulator on the genetic regulation of the tyramine cluster.

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