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Domain I and II from newly emerging goose tembusu virus envelope protein functions as a dominant-negative inhibitor of virus infectivity

文献类型： 外文期刊

作者： Zhao, Dongmin 1 ; Huang, Xinmei 1 ; Liu, Yuzhuo 1 ; Han, Kaikai 1 ; Zhang, Jingfeng 1 ; Yang, Jing 1 ; Xie, Xingxing 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Minist Agr, Key Lab Vet Biol Engn & Technol, Nanjing 210014, Jiangsu, Peoples R China

关键词： Goose tembusu virus;Envelope protein;Domain land II;Inhibition

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

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收录情况： SCI

摘要： Flavivirus envelope protein locates at the outermost surface of viral particle and mediates virus entry and fusion infection, and domains I and II of E protein play an important role in this process. In this study, we have expressed and purified goose tembusu virus (GTV) E protein domains I and II (DI/II) from E. coli, and tested conceptual approach that purified protein serves as anti-viral reagent. We found that DI/II inhibited GTV JS804 infection in BHK-21 cells in a dose-dependent manner, and this inhibition activity was achieved by binding to cell membrane specifically. Moreover, JS804 treated with DI/II specific antiserum decreased its infectivity to BHK-21 cells. Taken together, this is first to show that the purified DI/ II domain of tembusu virus expressed in E. coli was able to interfere with virus infection, which opens an avenue to develop novel anti-viral regents to prevent and eventually eradicate GTV infection. (C) 2014 Elsevier Ltd. All rights reserved.

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