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Biochemical composition of the ovarian fluid and its effects on the fertilization capacity of turbot Scophthalmus maximus during the spawning season

文献类型: 外文期刊

作者: Jia, Y. D. 1 ; Niu, H. X. 2 ; Meng, Z. 1 ; Liu, X. F. 1 ; Lei, J. L. 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Qingdao Key Lab Marine Fish Breeding Biotechnol, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Inner Mongolia Univ Nationalities, Sch Anim Sci & Technol, Tongliao 028042, Peoples R China

关键词: enzymes;flatfish;inorganic ions;insemination;pH;protein

期刊名称:JOURNAL OF FISH BIOLOGY ( 影响因子:2.051; 五年影响因子:2.25 )

ISSN:

年卷期:

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收录情况: SCI

摘要: This study investigated the biochemical composition of ovarian fluid and its effect on the fertilization capacity of turbot Scophthalmus maximus during the spawning season. The fertilization rate and pH of ovarian fluid varied throughout the spawning season, with the highest values recorded at the mid-season. Positive correlations were found between the fertilization rate and the ovarian fluid pH. The composition of major inorganic ions (Na+, K+, Ca2+ and Cl-) showed no significant changes during the spawning season. Alkaline phosphatase (AKP) activity was significantly higher during mid-season than other seasons. The lowest levels of protein, acid phosphatase (ACP) and aspartate aminotransferase (AAT) were in the ovarian fluid released at the mid-season. Moreover, significant relationships were observed between the fertilization rate and the levels of protein, ACP, AKP and AAT. These observations suggest that the biochemical profile of ovarian fluid affects the insemination microenvironment as well as the fertilization capacity of S. maximus eggs. Determination of such profiles may prove to be a useful strategy to improve S. maximus breeding techniques.

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