Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara
文献类型： 外文期刊
作者： Liu, X. F. 1 ; Wu, Y. H. 1 ; Wei, S. N.; Wang, N. 1 ; Li, Y. Z. 1 ; Zhang, N. W. 1 ; Li, P. F.; Qin, Q. W.; Chen, S 1 ;
作者机构： 1.CAFS, Key Lab Sustainable Dev Marine Fisheries, Yellow Sea Fisheries Res Inst, Minist Agr, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China
3.Ocean Univ China, Coll Marine Life, Qingdao 266003
关键词： brain-cell line;GFP;LCDV;NNV;primary culture;SGIV
期刊名称：JOURNAL OF FISH BIOLOGY （ 影响因子：2.051； 五年影响因子：2.25 ）
ISSN： 0022-1112
年卷期： 2018 年 92 卷 2 期
页码：
收录情况： SCI
摘要： A new brain-cell line, EMB, was developed from kelp grouper Epinephelus moara, a cultured marine fish. The EMB cells were subcultured for more than 60 passages. The cells were cultured in Leibovitz's L-15 medium (L15) supplemented with antibiotics, foetal bovine serum (FBS), 2-mercaptoethanol (2-ME) and basic fibroblast growth factor (bFGF). The cells could grow at 18-30 degrees C, with the maximum growth between 24 and 30 degrees C. The optimum FBS concentration for the cells growth ranged between 15 and 20%. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 45. After being transfected with pEGFP-N3 plasmid, the cells could successfully express green fluorescence protein (GFP), implying that this cell line can be used for transgenic studies. A significant cytopathic effect (CPE) was observed in the cells after infection with Singapore grouper iridovirus (SGIV) or red spotted grouper nervous necrosis virus (RGNNV) and the viral replication was confirmed by quantitative real-time PCR (qrt-PCR) assay, which suggested EMB's application potential for studies of SGIV and RGNNV.
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