中国水产科学研究院机构知识库

MOLECULAR CHARACTERIZATION, POLYMORPHISM AND EXPRESSION ANALYSIS OF SWAMP EEL MAJOR HISTOCOMPATIBILITY COMPLEX CLASS II B GENE, AFTER INFECTION BY AEROMONAS HYDROPHILIA

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文献类型：外文期刊

作者： Li, W. ¹ ; Sun, W. X. ¹ ; Hu, J. F. ¹ ; Hong, D. W. ¹ ; Chen, S. L. ² ;

作者机构： 1.Yangtze Univ, Coll Life Sci, Jinzhou 434025, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao 266071, Peoples R China

关键词： Major histocompatibility complex (MHC);cDNA;genomic structure;polymorphism;swamp eel (Monopterus albus)

期刊名称：JOURNAL OF ANIMAL AND PLANT SCIENCES （影响因子：0.49；五年影响因子：0.686 ）

ISSN： 1018-7081

年卷期： 2014 年 24 卷 2 期

页码：

收录情况： SCI

摘要： Major histocompatibility complex (MHC) class II genes play a crucial role in the immune system of vertebrates. Here we report the cloning of full-length cDNA from the swamp eel (Monopterus albus) MHC class II B (MHC-DAB) gene. We described its genomic structure, molecular polymorphism and expression profiles. The full-length cDNA (Gen Bank accession No.:JQ236680) is 1339 bp, encoding a 249 amino acid peptide. The genomic sequence was identified to be 3066 bp in length, which contained six exons and five introns. Sequence comparison showed that the proposed amino acid sequence shared between 26.4 and 74.7% identity with other species. Thirty seven distinct alleles were isolated from sixty individuals. There were between two and five alleles per individual. The presence of five alleles in an individual suggested that there were at least three MHC IIB loci in the genome. At the protein-binding region d(N) was significantly greater than d(S), providing evidence of strong positive selection among swamp eel sequences. RT-PCR demonstrated high MHC-DAB expression in stomach, spleen, blood cell and liver, moderate expression in brain, skin and kidney, and low or negligible expression in heart, intestine and muscle. Great changes were observed in liver, spleen and intestine after challenged with pathogenic bacteria, Aeromonas hydrophilia.

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MOLECULAR CHARACTERIZATION, POLYMORPHISM AND EXPRESSION ANALYSIS OF SWAMP EEL MAJOR HISTOCOMPATIBILITY COMPLEX CLASS II B GENE, AFTER INFECTION BY AEROMONAS HYDROPHILIA

作者其他论文 更多>>

Identifying the germ cells during embryogenesis and gametogenesis by germ-line gene vasa in an anadromous fish, American shad Alosa sapidissima

Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara

Development of microsatellite markers and genetic diversity analysis for Pelodiscus sinensis

Estimation of genetic parameters for disease-resistance traits in Cynoglossus semilaevis (Gunther, 1873)

Special collector and count method in a recirculating aquaculture system for calculation of feed conversion ratio in fish

Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossus semilaevis)

Polymorphic dinucleotide microsatellites in tongue sole (Cynoglossus semilaevis)

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