中国水产科学研究院机构知识库

Polymorphic dinucleotide microsatellites in tongue sole (Cynoglossus semilaevis)

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文献类型：外文期刊

作者： Liao, X. ¹ ; Shao, C. W. ¹ ; Tian, Y. S. ¹ ; Chen, S. L. ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China

关键词： Cynoglossus semilaevis;enriched genomic library;microsatellites

期刊名称：MOLECULAR ECOLOGY NOTES （影响因子：2.384；五年影响因子：2.551 ）

ISSN： 1471-8278

年卷期： 2007 年 7 卷 6 期

收录情况： SCI

摘要： The tongue sole, Cynoglossus semilaevis, is a rare marine flatfish distributed in Chinese coastal waters. From a (GT)(n)-enriched genomic library, 57 microsatellites were isolated and characterized. Seventeen of these loci were polymorphic in a test population with alleles ranging from three to 13, and observed and expected heterozygosities from 0.1613 to 1.0000 and from 0.2126 to 0.8983, respectively. Five loci deviated from the Hardy-Weinberg equilibrium in the sampled population, and linkage disequilibrium between two loci was significant after applying Bonferroni correction. Three additional fish species assessed for cross-species amplification revealed that only one locus was also polymorphic in one species. These polymorphic microsatellite loci should provide sufficient level of genetic diversity to evaluate the breeding strategy and investigate the fine-scale population structure in C. semilaevis.

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