您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Visual and microplate detection of aflatoxin B2 based on NaCl-induced aggregation of aptamer-modified gold nanoparticles

文献类型： 外文期刊

作者： Luan, Yunxia 1 ; Chen, Jiayi 1 ; Xie, Gang 2 ; Li, Cheng 1 ; Ping, Hua 1 ; Ma, Zhihong 1 ; Lu, Anxiang 1 ;

作者机构： 1.Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China

2.Acad State Adm Grain, Beijing 100037, Peoples R China

关键词： Aflatoxin B2;Aptamer;Gold nanoparticles;Colorimetry;Mycotoxin

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： We describe a fast and sensitive method for the detection of aflatoxin B2 (AFB2) that is based on the addition of AFB2-sensitive aptamers to a colloidal solution of gold nanoparticles (AuNPs). Subsequent addition of AFB2 and NaCl to the solution causes a color change from wine-red to blue-purple. Best results are obtained at a pH value of 7.0, a 10 mM aptamer concentration, and a 2 M concentration of NaCl. The method has a linear dynamic range that extends from 0.025 to 10 nga (TM) mL(-1) of AFB2, and the detection limit is 25 pga (TM) mL(-1). The method is simple, fast, highly sensitive, and enables both visual and microplate readout.

  • 相关文献

[1]Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles. Luan, Yunxia,Chen, Jiayi,Li, Cheng,Fu, Hailong,Ma, Zhihong,Lu, Anxiang,Luan, Yunxia,Chen, Jiayi,Li, Cheng,Fu, Hailong,Ma, Zhihong,Lu, Anxiang,Xie, Gang. 2015

[2]Rapid Visual Detection of Aflatoxin B1 by Label-Free Aptasensor Using Unmodified Gold Nanoparticles. Luan, Yunxia,Chen, Jiayi,Lu, Anxiang,Li, Cheng,Fu, Hailong,Ma, Zhihong,Wang, Jihua,Chen, Zhengbo,Xie, Gang.

[3]An oligosorbent-based aptamer affinity column for selective extraction of aflatoxin B-2 prior to HPLC with fluorometric detection. Liu, Hongmei,Luan, Yunxia,Lu, Anxiang,Li, Bingru,Wang, JiHua,Yang, Meihua,Yang, Meihua. 2018

[4]A Label-Free Aptamer-Based Fluorescent Assay for Cadmium Detection. Luan, Yunxia,Lu, Anxiang,Chen, Jiayi,Fu, Hailong,Xu, Li,Luan, Yunxia,Lu, Anxiang,Chen, Jiayi,Fu, Hailong,Xu, Li. 2016

[5]Superior fluorescent probe for detection of potassium ion. Chen, Zhengbo,Tan, Lulu,Hu, Liangyu,Luan, Yunxia.

[6]Chemiluminescence assay for detection of 2-hydroxyfluorene using the G-quadruplex DNAzyme-H2O2-luminol system. Liang, Gang,Man, Yan,Li, An,Jin, Xinxin,Pan, Ligang,Liang, Gang,Man, Yan,Li, An,Jin, Xinxin,Pan, Ligang,Liang, Gang,Man, Yan,Li, An,Jin, Xinxin,Pan, Ligang,Liang, Gang,Liu, Xinhui. 2018

[7]Aptamer-based biosensor for label-free detection of ethanolamine by electrochemical impedance spectroscopy. Liang, Gang,Man, Yan,Jin, Xinxin,Pan, Ligang,Liang, Gang,Man, Yan,Jin, Xinxin,Pan, Ligang,Liang, Gang,Man, Yan,Jin, Xinxin,Pan, Ligang,Liang, Gang,Liu, Xinhui.

[8]Recent Advances in Mycotoxin Determination for Food Monitoring via Microchip. Man, Yan,Liang, Gang,Li, An,Pan, Ligang,Man, Yan,Liang, Gang,Li, An,Pan, Ligang,Man, Yan,Liang, Gang,Li, An,Pan, Ligang. 2017

[9]Characterization of Phenolic Compounds from Early and Late Ripening Sweet Cherries and Their Antioxidant and Antifungal Activities. Wang, Meng,Jiang, Nan,Wang, Yao,Jiang, Dongmei,Feng, Xiaoyuan,Wang, Meng,Jiang, Nan,Wang, Yao,Jiang, Dongmei,Feng, Xiaoyuan.

[10]A single-step solid phase extraction for the simultaneous determination of 8 mycotoxins in fruits by ultra-high performance liquid chromatography tandem mass spectrometry. Wang, Meng,Jiang, Nan,Xian, Hong,Wei, Dizhe,Feng, Xiaoyuan,Wang, Meng,Jiang, Nan,Xian, Hong,Wei, Dizhe,Feng, Xiaoyuan,Shi, Lei.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台