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Microcystin-RR exposure results in growth impairment by disrupting thyroid endocrine in zebrafish larvae

文献类型： 外文期刊

作者： Xie, Liqiang 1 ; Yan, Wei 2 ; Li, Jing 3 ; Yu, Liqin 3 ; Wang, Jianghua 3 ; Li, Guangyu 3 ; Chen, Nan 3 ; Steinman, Alan 1 ;

作者机构： 1.Chinese Acad Sci, Nanjing Inst Geog & Limnol, State Key Lab Lake Sci & Environm, Nanjing 210008, Jiangsu, Peoples R China

2.Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Wuhan 430064, Peoples R China

3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China

4.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China

5.Grand Valley State Univ, Robert B Annis Water Resources Inst, Muskegon, MI 49441 USA

关键词： MC-RR;Developing zebrafish;Growth impairment;Hypothalamic-pituitary-thyroid axis

期刊名称：AQUATIC TOXICOLOGY （ 影响因子：4.964； 五年影响因子：5.071 ）

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收录情况： SCI

摘要： Recent studies have shown that cyanobacteria-derived microcystins (MCs) have the potential to disrupt endocrine systems. However, the effects of microcystin-RR (MC-RR) and their underlying mechanisms are poorly resolved in fish. In this study, MC-RR exposure through submersion caused serious developmental toxicity, such as growth delay and depressed heart rates in zebrafish larvae. We also detected decreased levels of thyroid hormones (THs), suggesting that MC-RR-triggered thyroid endocrine disruption might contribute to the growth impairment observed in developing zebrafish. To further our understanding of mechanisms of MC-RR-induced endocrine toxicity, quantitative real-time PCR (QPCR) analysis was performed on hypothalamic-pituitary-thyroid (HPT) axis related genes, i.e., corticotropin-releasing factor (CRF), thyroid-stimulating hormone (TSH), sodium/iodide symporter (NIS), thyroglobulin (TG), thyroid receptors (TR alpha and TR beta) and iodothyronine deiodinases (Dio1 and Dio2), of developing zebrafish embryos exposed to 0, 0.3, 1.0 or 3.0 mg L-1 MC-RR until 96 h post-fertilization. Our results showed that transcription pattern of HPT axis related genes were greatly changed by MC-RR exposure, except TG gene. Furthermore, western blot was used to validate the results of gene expression. The results showed protein synthesis of TG was not affected, while that of NIS was significantly up-regulated, which are in accordance with gene expression. The overall results indicated that exposure to MC-RR can induce developmental toxicity, which might be associated with thyroid endocrine disruption in developing zebrafish larvae. (C) 2015 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Waterborne exposure to microcystin-LR alters thyroid hormone levels and gene transcription in the hypothalamic-pituitary-thyroid axis in zebrafish larvae. Chen, Jun,Yan, Wei,Zhou, Youxiang,Yang, Jie,Li, Shuqian,Hu, Dingjin,Wang, Jianghua,Li, Guangyu. 2012

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