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Two proteins of Cucurbit chlorotic yellows virus, P59 and P9, are self-interacting

文献类型： 外文期刊

作者： Wang, Zhenyue 1 ; Wang, Yingzhi 1 ; Sun, Hu 2 ; Gu, Qinsheng 3 ; Li, Honglian 1 ; Sun, Bingjian 1 ; Shi, Yajuan 1 ; Shi, 1 ;

作者机构： 1.Henan Agr Univ, Coll Plant Protect, Zhengzhou 450002, Peoples R China

2.Henan Acad Agr Sci, Inst Plant Protect, Zhengzhou 450002, Peoples R China

3.Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Zhengzhou 450009, Peoples R China

关键词： Crinivirus;CCYV;Y2HS;Interaction

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

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收录情况： SCI

摘要： The yeast two-hybrid (Y2H) assay, a powerful tool for identifying protein-protein interactions, has been widely used to study viral protein interactions and to elucidate the functions of viral proteins. In this study, Cucurbit chlorotic yellows virus-encoded proteins were investigated by Y2H assays in all possible pairwise combinations, and the self-interactions of P59 and P9 were detected. The interacting domains of P59 and P9 were identified using vectors carrying an activation domain fused to a truncated version of P59 or P9. We found that the middle region (amino acids 173-344) of P59 was necessary for this self-interaction, while three different truncated versions of P9 showed no interaction with full-length P9. This is the first report of the self-interaction of P59 in the genus Crinivirus,

  • 相关文献

[1]One-step reverse transcription loop mediated isothermal amplification assay for sensitive and rapid detection of Cucurbit chlorotic yellows virus. Wang, Zhenyue,Li, Honglian,Sun, Bingjian,Liang, Xiangzhi,Yuan, Yuan,Liu, Ruili,Shi, Yan,Sun, Hu,Gu, Qinsheng. 2014

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