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Ultrasensitive immunoassays based on biotin-streptavidin amplified system for quantitative determination of family zearalenones

文献类型： 外文期刊

作者： Liu, Na 2 ; Nie, Dongxia 3 ; Zhao, Zhiyong 3 ; Meng, Xianjun 2 ; Wu, Aibo 1 ;

作者机构： 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab Food Safety Res, Shanghai 200031, Peoples R China

2.Shenyang Agr Univ, Coll Food Sci, Shenyang 110161, Liaoning, Peoples R China

3.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, Minist Agr, Lab Qual & Safety Risk Assessment Agroprod Shangh, Shanghai 201403, Peoples R China

关键词： Zearalenones (ZENs);Biotin-streptavidin system (BSAS);ELISA;FLISA

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

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收录情况： SCI

摘要： In this work, based on a newly obtained monoclonal antibody (MAb) against zearalenone (ZEN) and biotin-streptavidin system (BSAS) for signal amplification, two sensitive and rapid immunoassay formats including biotin-streptavidin amplified enzyme-linked immunosorbent assay (BA-ELISA) and biotin-streptavidin amplified fluorescence-linked immunosorbent assay (BA-FLISA), were developed for family zearalenones (ZENs) determination. And the limits of detection (LODs) of ZEN were 0.02 ng mL(-1) and 0.10 ng mL(-1) for BA-ELISA and BA-FLISA respectively. Using the BA-ELISA platform the half maximal inhibitory concentrations (IC50) were 0.18 ng mL(-1) for ZEN, 0.39 ng mL(-1) for alpha-zearalenol (alpha-ZOL), 0.46 ng mL(-1) for beta-zearalenol (beta-ZOL), 0.30 ng mL(-1) for zearalanone (ZAN), 0.30 ng mL(-1) for alpha-zearalanol (alpha-ZAL), and 0.73 ng mL(-1) for beta-zearalanol (beta-ZAL). With the broad specificity, the developed immunoassays could be used as sensitive and valuable tools for detection of family ZENs. Additionally, the suitability of the proposed immunoassays for its application to corn flour and corn based baby food has also been investigated. (C) 2015 Elsevier Ltd. All rights reserved.

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