One-step green synthesis of a polypyrrole-Au nanocomposite and its application in myoglobin aptasensor
文献类型： 外文期刊
作者： Sun, Chong 1 ; Wang, Daoying 1 ; Geng, Zhiming 1 ; Gao, Ling 2 ; Zhang, Muhan 1 ; Bian, Huan 1 ; Liu, Fang 1 ; Zhu, Yongz 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing 210014, Jiangsu, Peoples R China
2.Nanjing Normal Univ, Coll Chem & Mat Sci, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Nanjing 210023, Jiangsu, Peoples R China
期刊名称：ANALYTICAL METHODS （ 影响因子：2.896； 五年影响因子：2.716 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： An electrochemical aptasensor was fabricated based on a polypyrrole-Au nanocomposite (PPy-Au NC) and myoglobin-binding aptamer (MBA). The PPy-Au NC was synthesized using a one-step green synthesis method and was characterized by transmission electron microscopy (TEM), UV-vis spectroscopy and a diffuse reflectance spectrum. The studies revealed that the PPy-Au NC provided a mild micro-environment and large surface area for MBA immobilization and facilitated electron transfer. Glassy carbon electrodes (GCEs) surface modified with PPy-Au NC were grafted with MBA, which had excellent binding affinity and selectivity for myoglobin (Mb). The binding of Mb at the modified GCE surface greatly restrained the access of electrons for a redox probe of [Fe(CN)(6)] (3-/4) . Moreover, the aptasensor could be used for the detection of Mb in biochemical assays, with a wide detection range (0.0001 to 0.15 g L-1) and a low detection limit of 30.9 ng mL(-1). The aptasensor had a good anti-interference property towards hemin, glucose oxidase (GOx), cytochrome c and hemoglobin. The idea and method presented herein provide a new approach for the evaluation of the freshness and quality of stored meat.
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