您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effective inhibition of porcine epidemic diarrhea virus by RNA interference in vitro

文献类型： 外文期刊

作者： Shen, Haiyan 1 ; Zhang, Chunhong 1 ; Guo, Pengju 2 ; Liu, Zhicheng 1 ; Zhang, Jianfeng 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangdong Open Lab Vet Publ Hlth, Guangdong Prov Key Lab Livestock Dis Prevent, Guangzhou 510640, Guangdong, Peoples R China

2.Guangdong Lab Anim Monitoring Inst, Guangzhou 510640, Guangdong, Peoples R China

关键词： Porcine epidemic diarrhea virus;RNA interference;Short hairpin RNA

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV) is a member of the coronaviridae family, which can cause acute and highly contagious enteric disease of swine characterized by severe entero-pathogenic diarrhea in piglets. Currently, the vaccines of PEDV are only partially effective and there is no specific drug available for treatment of PEDV infection. To exploit the possibility of using RNA interference (RNAi) as a strategy against PEDV infection, five shRNA-expressing plasmids targeting the N, M, and S genes of PEDV were constructed and transfected into Vero cells. The cytopathic effect and MTS assays demonstrated that two shRNAs (pSilencer4.1-M1 and pSilencer4.1-N) were capable of protecting cells against PEDV invasion with very high specificity and efficiency. The two shRNA expression plasmids were also able to inhibit the PEDV replication significantly, as shown by detection of virus titers (TCID50/mL). A real-time quantitative RT-PCR further confirmed that the amounts of viral RNAs in cell cultures pre-transfected with these two plasmids were reduced by 95.0 %. Our results suggest that RNAi might be a promising new strategy against PEDV infection.

  • 相关文献

[1]Characterization of the porcine epidemic diarrhea virus codon usage bias. Chen, Ye,Shi, Yuzhen,Deng, Hongjuan,Gu, Ting,Xu, Jian,Ou, Jinxin,Jiang, Zhiguo,Jiao, Yiren,Zou, Tan,Wang, Chong.

[2]Molecular cloning and virus-induced gene silencing of MiASB in the southern root-knot nematode, Meloidogyne incognita. Huang, Yonghong,Huang, Yonghong,Mei, Mei,Mao, Zhenchuan,Xie, Bingyan,Mei, Mei,Lv, Shun,Zhou, Jiankun,Chen, Shi. 2014

[3]Expressing p20 hairpin RNA of Citrus tristeza virus confers Citrus aurantium with tolerance/resistance against stem pitting and seedling yellow CTV strains. Bei Xue-jun,Zhang Yong-yan,Lu Zhi-ming,Cheng Chun-zhen,Bei Xue-jun,Zhang Yong-yan,Zhong Guang-yan,Yang Jia-wei,Yan Hu-bin,Cheng Chun-zhen,Zhong Guang-yan. 2015

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台