A gamma-Glutamylcysteine Synthetase Gene from Pyrus calleryana Is Responsive to Ions and Osmotic Stresses
文献类型： 外文期刊
作者： Li, Hui 1 ; Han, Jin-long 1 ; Lin, Jing 1 ; Yang, Qing-song 1 ; Chang, You-hong 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Hort, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Jiangsu, Peoples R China
关键词： Expression regulation;Glutathione content;Pyrus calleryana;Stress tolerance;gamma-Glutamylcysteine synthetase
期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Pyrus calleryana Decne. is widely used as a pear rootstocks in Asia. Glutathione (GSH) can induce the phytochelatin synthase gene's (PcPCS1) transcription in this plant when exposed to cadmium. This phenomenon indicated that GSH plays a vital role in protecting P. calleryana from cadmium stress. Here, we have isolated and characterized a gene encoding.-glutamylcysteine synthetase (Pc gamma ECS), the key enzyme of GSH biosynthesis, from the leaves of P. calleryana, with the aim of expanding our knowledge of the role of GSH at the molecular level. P. calleryana plants treated with cadmium, salt, PEG6000, or mannitol responded by increasing transcription of the Pc gamma ECS gene. The response was specific for cadmium, which causes a toxicity that is thought to be mitigated through phtochelatins, and other treatments that cause damage that can be relieved by the synthesis of GSH. Feeding experiments suggested that cysteine contributed to activating the transcription of Pc gamma ECS and GSH synthesis. However, the gene's expression was inhibited in the presence of GSH. Recombinant Escherichia coli carrying Pc gamma ECS had higher GSH synthesis levels and grew better than the control cells under cadmium, salt, or osmotic stresses. These results suggest that Pc gamma ECS may participate in responses against multiple environmental stimuli in P. calleryana.
- 相关文献
作者其他论文 更多>>
-
Kynurenine, a derivative of tryptophan, inhibits progesterone biosynthesis in porcine granulosa luteal cells through Aryl hydrocarbon receptor-mediated downregulation of GATA-binding protein 4, 6, and CCAAT/enhancer-binding protein beta†
作者：Liao, Shiying;Deng, Yanfei;Liao, Shiying;Cheng, Jinhua;Zhao, Weimin;Dai, Chaohui;Fu, Yanfeng;Li, Bixia;Li, Hui;Li, Hui
关键词：kynurenine; progesterone; granulosa luteal cell; STAR; AHR
-
The Erk1/2-EGR1 signaling pathway is involved in lipopolysaccharide-induced transforming growth factor-beta 1 expression in mouse macrophages
作者：Zhao, Weimin;Wang, Hong;Dai, Chaohui;Li, Bixia;Fu, Yanfeng;Cheng, Jinhua;Li, Hui;Wang, Hong;Li, Hui
关键词：LPS; TGF-beta 1; Erk1/2; EGR1; Macrophage
-
Chlorogenic acid alleviates deoxynivalenol-induced damage in porcine trophectoderm cells by regulating the PI3K/AKT signaling pathway†
作者：Dai, Chaohui;Cheng, Jinhua;Li, Hui;Zhao, Weimin;Li, Bixia;Fu, Yanfeng;Wang, Xuemin;Liao, Chao;Yan, Junshu;Dai, Chaohui;Cheng, Jinhua;Li, Hui;Zhao, Weimin;Li, Bixia;Fu, Yanfeng;Wang, Xuemin;Liao, Chao;Yan, Junshu;Chen, Yanyu
关键词：pig; placental trophoblast cells; deoxynivalenol; chlorogenic acid; cell damage
-
Comprehensive Evolutionary Analysis of the SMXL Gene Family in Rosaceae: Further Insights into Its Origin, Expansion, Diversification, and Role in Regulating Pear Branching
作者：Liu, Chunxiao;Liu, Susha;Li, Hui;Wang, Zhonghua;Kan, Jialiang;Yang, Qingsong;Li, Xiaogang;Jiang, Xianda;Li, Xiaogang;Liu, Yilong;Li, Xiaogang
关键词：genome-wide identification; gene expansion; pear branching; Rosaceae; SMXLs
-
A Novel Allele in the Promoter of Wx Decreases Gene Expression and Confers Lower Apparent Amylose Contents in Japonica Rice (Oryza sativa L.)
作者：Tang, Weijie;Chen, Haiyuan;Zhang, Suobing;Tang, Jun;Lin, Jing;Fang, Xianwen;Zhang, Yunhui;Tang, Weijie;Chen, Haiyuan;Zhang, Suobing;Tang, Jun;Lin, Jing;Fang, Xianwen;Zhang, Yunhui;Chen, Gaoming;Zhang, Yunhui
关键词：Wx; promoter; allele; AAC; japonica rice; nitrogen
-
Integrated Methylome and Transcriptome Analysis between Wizened and Normal Flower Buds in Pyrus pyrifolia Cultivar 'Sucui 1'
作者：Li, Hui;Liu, Chunxiao;Kan, Jialiang;Lin, Jin;Li, Xiaogang
关键词：pear; wizened bud; methylation feature; auxin; cytokinin
-
Berberine alleviates inflammation in polycystic ovary syndrome by inhibiting hyaluronan synthase 2 expression
作者：He, Shaojing;Zhang, Qianjie;Li, Wei;Yang, Jing;Li, Saijiao;He, Shaojing;Li, Hui;Zhang, Qianjie;Zhao, Weimin;Dai, Chaohui;Li, Bixia;Cheng, Jinhua;Li, Hui;Zhao, Weimin;Dai, Chaohui;Li, Bixia;Cheng, Jinhua;Wu, Shuang;Zhou, Zhongming
关键词：HAS2; Inflammation; Granulosa cell; Berberine; PCOS
