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Loss of Function of the Carotenoid Isomerase Gene BrCRTISO Confers Orange Color to the Inner Leaves of Chinese Cabbage (Brassica rapa L. ssp pekinensis)

文献类型： 外文期刊

作者： Su, Tongbing 1 ; Yu, Shuancang 1 ; Zhang, Jiao Wang Fenglan 2 ; Yu, Yangjun 1 ; Zhang, Deshuang 1 ; Zhao, Xiuyun 1 ; W 1 ;

作者机构： 1.BAAFS, BVRC, Beijing 100097, Peoples R China

2.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China

关键词： Brassica rapa L. ssp pekinensis;Orange leaf;Br-or;BrCRTISO;Carotenoid biosynthesis

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

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收录情况： SCI

摘要： Chinese cabbage varieties with orange inner leaves are of interest due to their attractive appearance and high carotenoid content. Previously, a recessive locus, Br-or, that confers this trait was mapped to linkage group A09 in Brassica rapa, and Bra031539 was identified as the candidate gene. We used a backcross population consisting of 1,392 individuals for fine mapping; three linked markers delimited the Br-or locus to a region of 9.47 kb, which contained only one predicted functional gene, Bra031539 (the carotenoid isomerase gene BrCRTISO). We identified two bacterial artificial chromosome (BAC) clones carrying Br-or and confirmed that this allele of BrCRTISO confers the mutant phenotype by sequencing. Many DNA sequence variations are present in Br-or, including a 90-bp DNA deletion in the promoter and a 501-bp insertion at the 3' end of the gene. GUS staining showed that the spatial expression of BrCRTISO was perfectly coincident with the site of carotenoid synthesis and that the 90-bp deletion in the promoter slightly downregulated expression of the gene. Using an in vitro enzyme assay, we showed that the mutant Br-or protein cannot convert prolycopene to all-trans-lycopene. Light has a positive effect on the expression of carotenoid biosynthesis genes, except for beta-ring carotene hydroxylase (CHXB), and this coincided with the increase in total lycopene and beta-carotene in the Br-or mutant. We also demonstrated that loss of BrCRTISO function, upregulation of the upstream genes, and downregulation of downstream genes lead to the accumulation of prolycopene and confer an orange color to the inner head leaves in Chinese cabbage.

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[1]Inheritance of downy mildew resistance at different developmental stages in Chinese cabbage via the leaf disk test. Zhang, Shengju,Yu, Shuancang,Zhang, Fenglan,Yu, Yangjun,Zhao, Xiuyun,Zhang, Deshuang,Wang, Weihong,Zhang, Shengju,Si, Longting. 2012

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