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Cloning and expression of a wild eggplant cytochrome P450 gene, StoCYP77A2, involved in plant resistance to Verticillium dahliae

文献类型: 外文期刊

作者: Yang, Liu 1 ; Shi, Ce 1 ; Mu, Xiaoying 1 ; Liu, Chao 1 ; Shi, Ke 1 ; Zhu, Wenjiao 1 ; Yang, Qing 1 ;

作者机构: 1.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China

关键词: P450;Tobacco;Solanum torvum;StoCYP77A2;Transformation;Verticillium wilt

期刊名称:PLANT BIOTECHNOLOGY REPORTS ( 影响因子:2.01; 五年影响因子:1.907 )

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收录情况: SCI

摘要: Plant P450s play an important role in plant response to environmental stimuli, especially to pathogen challenge, besides leading to the biosynthesis of secondary metabolites. In this work, a Verticillium dahliae-induced cDNA sequence was cloned from wild eggplant Solanum torvum and designated StoCYP77A2. StoCYP77A2 encodes a 511-amino acid peptide that shows sequence homology to the plant CYP77 family. StoCYP77A2 is expressed in a tissue-specific manner with the highest expression level in stem. The expression level of StoCYP77A2 was increased in wild eggplants which were infected with Verticillium dahlia. We expressed StoCYP77A2 in V. dahliae-sensitive tobacco, and the transgenic tobacco plants were more resistant to V. dahliae infection than the wild type. Protein extraction of transgenic tobacco showed strong antifungal activity against V. dahliae, indicating that StoCYP77A2 may be involved in the synthesis of some antimicrobial compounds. These results suggest that StoCYP77A2 plays a role in plant defense to V. dahliae infection.

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