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Immunological, ionic and biochemical responses in blood serum of the marine fish Trachinotus ovatus to poly-infection by Cryptocaryon irritans

文献类型: 外文期刊

作者: Yin, Fei 1 ; Sun, Peng 1 ; Tang, Baojun 1 ; Dan, Xueming 2 ; Li, Anxing 3 ;

作者机构: 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Minist Agr, Key Lab East China Sea & Ocean Fishery Resources, Shanghai 200090, Peoples R China

2.South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

3.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Key Lab Aquat Prod Safety,Minist Educ, Guangzhou 510275, Guangdong, Peoples R China

关键词: Cryptocaryon irritans;Trachinotus ovatus;Poly-infection;Protective immunity;Biochemical status

期刊名称:EXPERIMENTAL PARASITOLOGY ( 影响因子:2.011; 五年影响因子:2.132 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: To investigate the response of pompano fish (Trachinotus ovatus) to white spot disease, we used the protozoan Cryptocaryon irritans to infect live 450-g specimens at concentrations of 40,000 theronts/fish. We assessed the relative infection intensity (RII), serum immobilizing titer, and immunity-related enzyme activities (ACP, AMP, LZM), and assessed feeding, serum ion concentrations (Na+, Cl-, Ca2+ and K+) and blood biochemistry (ALT, AST, LDH) of pompano. The fish were then treated with a lethal dose of C irritans (70,000 theronts/fish) and the number of deaths was recorded. We found that the relative infection intensities of the control group, group I, and group II were 0, 0.630 +/- 0.179, and 0.014 +/- 0.006. Poly-infection induced a significant increase in the serum immobilizing titer (853.33 +/- 295.60) of group II. In terms of the biochemical assessment, group II had significantly higher alkaline phosphatase and acid phosphatase activities than the other groups, and the lowest lysozyme activity (P < 0.05), compared to higher activity in the control group and the highest level in group I. Only the fishes of group I had stopped feeding after treatment. The concentrations of Na+, Cl-, and Ca2+ in blood serum did not differ significantly among the three groups, but K+ concentration increased with the increasing infection frequency. Alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase activities in fish of group II were significantly higher than those of the other groups. Survival of the fish subjected to the lethal dose of C. irritans was 0, 0, and 100 in groups control, I, and II, respectively. In conclusions, based on the food intake of group II, along with the results of relative infection intensity, serum immobilizing titer, and survival, we speculate that the fish in that group acquired high protective immunity following poly-infection by C irritans, experiencing limited harm for pompano. (C) 2015 Elsevier Inc. All rights reserved.

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