DNA Methylation Changes Induced in Rice by Exposure to High Concentrations of the Nitric Oxide Modulator, Sodium Nitroprusside
文献类型: 外文期刊
作者: Ou, Xiufang 1 ; Zhuang, Tingting 1 ; Yin, Wenchao 1 ; Miao, Yiling 1 ; Wang, Bo 1 ; Zhang, Yunhong 1 ; Lin, Xiuyun 1 ; Xu 1 ;
作者机构: 1.NE Normal Univ, Key Lab Mol Epigenet MOE, Changchun, Peoples R China
2.Chinese Acad Sci, Changchun Inst Appl Chem, Changchun 130022, Peoples R China
3.Jilin Acad Agr Sci, Changchun, Peoples R China
4.Washington State Univ, Sch Mol Biosci, Dept Crop & Soil Sci, Pullman, WA 99164 USA
5.Washington State Univ, Ctr Reprod Biol, Pullman, WA 99164 USA
6.Washington State Univ, Ctr Reprod Bi
关键词: Nitric oxide;DNA methylation;Gene expression;Rice
期刊名称:PLANT MOLECULAR BIOLOGY REPORTER ( 影响因子:1.595; 五年影响因子:2.042 )
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年卷期:
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收录情况: SCI
摘要: Nitric oxide (NO) is a signaling molecule that participates at physiological concentrations in diverse regulatory pathways. However, when NO concentration reaches levels higher than normal, it is sensed as a stress, which results in epigenetic alterations and chromatin remodeling in plants. Here, we showed that exposure to 0.5 mM of sodium nitroprusside (SNP), a NO donor, induces stress symptoms in rice seedlings that result in complete growth inhibition. Moreover, these stress symptoms in SNP-treated plants were found to be associated with methylation changes in genomic DNA, which predominantly involve hypomethylation at the CHG sites. Concomitant with these changes, transcriptional activation of a number of genes and transposable elements (TEs) was also observed. Further analysis revealed that expression of four genes, Chromomethylase 3 (OsCMT3), deficient in DNA methylation 1 (OsDDM1a), OsDDM1b, and DEMETER (OsDME), involved in chromatin remodeling and DNA methylation homoeostasis have been specifically perturbed.
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