您好，欢迎访问贵州省农业科学院 机构知识库！
贵州省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

PROTEOMIC ANALYSIS OF UBIQUITINATED PROTEINS FROM DELTAMETHRIN-RESISTANT AND SUSCEPTIBLE STRAINS OF THE DIAMONDBACK MOTH, Plutella Xylostella L.

文献类型： 外文期刊

作者： Cheng, Luogen 1 ; Du, Yaqiong 1 ; Hu, Junli 1 ; Jiao, Dongxu 1 ; Li, Jin 1 ; Zhou, Zhou 1 ; Xu, Qin 2 ; Li, Fengliang 3 ;

作者机构： 1.Nanjing Normal Univ, Sch Life Sci, Jiangsu Key Lab Biodivers & Biotechnol, Nanjing 210023, Jiangsu, Peoples R China

2.Nanjing Normal Univ, Sch Energy & Mech Engn, Nanjing 210023, Jiangsu, Peoples R China

3.Guizhou Acad Agr Sci, Inst Plant Protect, Guiyang, Peoples R China

关键词： Plutella xylostella;ubiquitinated protein;stress;LC MS/MS;deltamethrin

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Ubiquitin, a small protein consisting of 76 amino acids, acts in protein degradation, DNA repair, signal transduction, transcriptional regulation, and receptor control through endocytosis. Using proteomics, we compared the differentially ubiquitinated proteins between a deltamethrin-resistant (DR) strain and a deltamethrin-sensitive (DS) strain in third-instar larvae of the diamondback moth. We used polyubiquitin affinity beads to enrich ubiquitinated proteins and then performed one-dimensional SDS-PAGE separation and mass spectrometric identification. In the DR strain, We found 17 proteins that were upregulated (relative to the DS strain), including carbonic anhydrase family members, ADP ribosylation factor 102F CG11027-PA, protein kinase 61C, phospholipase A2, dihydrolipoamide dehydrogenase, tyrosine hydroxylase, and heat shock proteins, and five proteins that were downregulated in the DS strain, including carboxylesterase and DNA cytosine-5 methyltransferase. These results were also verified by qPCR. The differentially ubiquitinated proteins/enzymes were mainly responsible for protein binding, catalytic activity, and molecular transducer activity. These results improve our understanding of the relationship between protein ubiquitination and the deltamethrin stress response. (C) 2015 Wiley Periodicals, Inc.

  • 相关文献

[1]The identification and characterisation of a new deltamethrin resistance-associated gene, UBL40, in the diamondback moth, Plutella xylostella (L.). Zhang, Hong,Cheng, Chen,Jiao, Dongxu,Zhou, Zhou,Cheng, Luogen,Li, Fengliang.

[2]Up-regulated expression of Ran reveals its potential role to deltamethrin stress in Kc cells. Liu, Wei,Chi, Qingping,Hu, Junli,Cheng, Luogen,Xu, Qin,Li, Fengliang.

[3]IDENTIFICATION OF PROTEASOME ALPHA6 SUBUNIT ASSOCIATED WITH DELTAMETHRIN RESISTANCE IN Drosophila melanogaster Kc CELLS. Hu, Junli,Chi, Qingping,Liu, Wei,Cheng, Luogen,Xu, Qin,Li, Fengliang.

[4]Analysis of UB and L40 resistance related to deltamethrin in Drosophila kc cells. Chi, Qingping,Liu, Wei,Hu, Junli,Cheng, Luogen,Li, Fengliang,Cheng, Ying,Jing, Xuejian,Xu, Qin.

[5]Downregulation of ubiquitin gene expression in the deltamethrin-resistant diamondback moth (Plutella xylostella L.). Luogen, C.,Jianing, X.,Jing, X.,Ying, W.,Zhongying, L..

[6]Cloning and characterization of ubiquitin ribosome fusion gene RpS27a, a deltamethrin-resistance-associated gene from diamondback moth (Plutella xylostella L.). Zhang, Hong,Cheng, Chen,Gu, Shuyu,Zhou, Zhou,Cheng, Luogen,Li, Fengliang.

[7]cDNA Representational Difference Analysis of Deltamethrin-Resistant and -Susceptible Strains of Diamondback Moth. Zhang, Hong,Cheng, Chen,Liu, Bai-Duo,Cheng, Luo-Gen,Li, Feng-Liang. 2013

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：贵州省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台