文献类型: 外文期刊
作者: Zhang, Y. 1 ; Verhoeff, N. I. 1 ; Chen, Z. 5 ; Chen, S. 5 ; Wang, Mei 1 ; Zhu, Zhen 2 ; Ouwerkerk, P. B. F. 1 ;
作者机构: 1.Leiden Univ, Dept Mol & Dev Genet, Inst Biol IBL, NL-2300 RA Leiden, Netherlands
2.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
5.Fujian Acad Agr Sci, Biotechnol Res Inst, Fuzhou 350003, Fujian, Peoples R China
6.SU BioMed TNO Qual Life, NL-2301 CE Leiden, Netherlands
关键词: Dof;OsC4PPDK;Transcription factor;Rice
期刊名称:PLANT MOLECULAR BIOLOGY ( 影响因子:4.076; 五年影响因子:4.89 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Relative little is known about the functions of the so-called Dof zinc factors in plants. Here we report on the analysis of OsDof25 and show a function in regulation of the important C4 photosynthesis gene, OsC4PPDK in rice. Over-expression of OsDof25 enhanced the expression of OsC4PPDK in transient expression experiments by binding in a specific way to a conserved Dof binding site which was confirmed by yeast and in vitro binding studies. Expression studies using promoter GUS plants as well as qPCR experiments showed that OsDof25 expressed in different tissues including both photosynthetic and non-photosynthetic organs and that expression of OsDof25 was partially overlapping with the OsC4PPDK gene. Conclusive evidence for a role of OsDof25 in regulation of C4PPDK came from loss-of-function and gain-of-function experiments with transgenic rice, which showed that down-regulation or over-expression of OsDof25 correlated with OsC4PPDK expression and that OsDof25 has functions as transcriptional activator.
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