Depiction of carbohydrate-active enzyme diversity in Caldicellulosiruptor sp F32 at the genome level reveals insights into distinct polysaccharide degradation features
文献类型： 外文期刊
作者： Meng, Dong-Dong 1 ; Ying, Yu 1 ; Zhang, Kun-Di 1 ; Lu, Ming 1 ; Li, Fu-Li 1 ;
作者机构： 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
期刊名称：MOLECULAR BIOSYSTEMS （ 影响因子：3.336； 五年影响因子：2.986 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Thermophilic bacterium Caldicellulosiruptor sp. F32 can utilize cellulose-, hemicellulose-containing biomass, including unpretreated wheat straw. We have conducted a bioinformatics analysis of the carbohydrate-active enzyme (CAZyme) in the genome of Caldicellulosiruptor sp. F32, which reveals a broad substrate range of the strain. Among 2285 predicted open reading frames (ORFs), 73 (3.2%) CAZyme encoding genes, including 44 glycoside hydrolases (GHs) distributing in 22 GH families, 6 carbohydrate esterases (CEs), 3 polysaccharide lyases (PLs), 21 glycosyl transferases (GTs), and 25 carbohydrate-binding modules (CBMs) were found. An in-depth bioinformatics analysis of CAZyme families that target cellulose, hemicellulose, chitin, pectin, starch, and beta-1,3-1,4-glucan degradation were performed to highlight specialized polysaccharide degrading abilities of strain F32. A great number of orthologous multimodular CAZymes of Caldicellulosiruptor sp. F32 were found in other strains of genus Caldicellulosiruptor. While, a portion of the CAZymes of Caldicellulosiruptor sp. F32 showed sequence identity with proteins from strains of genus Clostridium. A thermostable beta-glucosidase BlgA synergistically facilitated the enzymatic degradation of Avicel by endo-1,4-beta-glucanase CelB, which indicated that the synchronous action of synergism between CAZymes enhanced the lignocellulose degradation by Caldicellulosiruptor sp. F32.
- 相关文献
作者其他论文 更多>>
-
Feasibility and mechanism of recycling carbon resources from waste cyanobacteria and reducing microcystin toxicity by dielectric barrier discharge plasma
作者：Wang, Jie;Cheng, Guofeng;Zhang, Jiahua;Liu, Xingguo;Wang, Jie;Cheng, Guofeng;Zhang, Jiahua;Liu, Xingguo;Shangguan, Yuyi;Lu, Ming
关键词：Short-chain fatty acids (SCFAs); Plasma technology; Advanced oxidation technology; Anaerobic fermentation; Reactive oxygen species (ROS)
-
Mechanism of dielectric barrier plasma technology to improve the quantity and quality of short chain fatty acids in anaerobic fermentation of cyanobacteria
作者：Wang, Jie;Liu, Xingguo;Shangguan, Yuyi;Lu, Ming;Xu, Junli
关键词：Algae; Anaerobic fermentation; Microcystin; Reactive oxygen species (ROS); Advanced oxidation technology
-
Perovskite LaMnO3 Composite Graphene Carbon Nitride g-C3N4 Improves the Photocatalytic Performance of Tetracycline Degradation
作者：Lu, Ming;Dong, Jiaqi;Hu, Mingzhu;Lv, Juan;Cheng, Guofeng
关键词：photocatalysis; LaMnO3/g-C3N4; visible light; z-scheme mechanism
-
Mechanism of dielectric barrier discharge plasma technology to improve the quantity of short-chain fatty acids in anaerobic fermentation of waste active sludge
作者：Wang, Jie;Liu, Xingguo;Cheng, Guofeng;Wang, Jie;Liu, Xingguo;Cheng, Guofeng;He, Jinling;Zhang, Ai;Xu, Junli;Lu, Ming;Shangguan, Yuyi
关键词：anaerobic digestion; microbial community structure; sludge recycling; sludge carbon resource; plasma technology
-
Evaluation of Trophic Structure and Energy Flow in a Pelteobagrus fulvidraco Integrated Multi-Trophic Aquaculture System
作者：Zhao, Yuxi;Liu, Xingguo;Lu, Ming;Zhou, Runfeng;Sun, Zhaoyun;Xiao, Shuwen;Zhao, Yuxi;Liu, Xingguo;Lu, Ming;Zhou, Runfeng;Sun, Zhaoyun;Xiao, Shuwen;Zhao, Yuxi;Zhou, Runfeng;Xiao, Shuwen;Lu, Ming;Sun, Zhaoyun
关键词：Pelteobagrus fulvidraco; integrated multi-trophic aquaculture; Ecopath model; nutrient structure; energy throughput
-
Characteristics of Bacterial Community in Pelteobagrus fulvidraco Integrated Multi-Trophic Aquaculture System
作者：Zhao, Yu-Xi;Liu, Xing-Guo;Lu, Ming;Zhou, Run-Feng;Sun, Zhao-Yun;Xiao, Shu-Wen;Zhao, Yu-Xi;Liu, Xing-Guo;Lu, Ming;Zhou, Run-Feng;Sun, Zhao-Yun;Xiao, Shu-Wen;Zhao, Yu-Xi;Zhou, Run-Feng;Xiao, Shu-Wen;Lu, Ming;Sun, Zhao-Yun
关键词：Pelteobagrus fulvidraco; integrated multi-trophic aquaculture; water quality; bacterial community
-
A Novel Glucose-Tolerant GH1 beta-Glucosidase and Improvement of Its Glucose updates Tolerance Using Site-Directed Mutation
作者：Sun, Jingjing;Wang, Wei;Hao, Jianhua;Sun, Jingjing;Wang, Wei;Hao, Jianhua;Ying, Yu;Hao, Jianhua
关键词：Marine bacterium; beta-Glucosidase; Glucose tolerance; Site-directed mutation; Ethanol tolerance
