您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Construction of Eimeria tenella multi-epitope DNA vaccines and their protective efficacies against experimental infection

文献类型： 外文期刊

作者： Song, Xiaokai 1 ; Xu, Lixin 1 ; Yan, Ruofeng 1 ; Huang, Xinmei 1 ; Li, Xiangrui 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Jiangsu, Peoples R China

关键词： Eimeria tenella;T-cell epitopes;DNA vaccine;Cytokine

期刊名称：VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY （ 影响因子：2.046； 五年影响因子：2.217 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The search for effective vaccines against chicken coccidiosis remains a challenge because of the complex organisms with multiple life cycle stages of Eimeria. Combination of T-cell epitopes from different stages of Eimeria life cycle could be an optimal strategy to overcome the antigen complexity of the parasite. In this study, 4 fragments with concentrated T-cell epitopes from the sporozoite antigen 507 and the merozoite antigen MZ5-7 of Eimeria tenella were cloned into eukaryotic expression vector pVAX1 in different forms, with or without chicken cytokines IL-2 or IFN-gamma genes as genetic adjuvants, to construct multistage, multi-epitope DNA vaccines against Eimeria tenella. Transcription and expression of the multi-epitope DNA vaccines in vivo were detected by reverse transcription-PCR (RT-PCR) and Western blot. On the basis of survival rate, lesion score, body weight gain, oocyst decrease ratio and the anti-coccidial index (ACI), Animal experiments were carried out to evaluate the protective efficacy against Eimeria tenella. Results showed the constructed DNA vaccines were transcribed and translated successfully in vivo. Animal experiment showed that the multi-epitopes DNA vaccines were more effective to stimulate immune response than single fragment. Compared with the DNA vaccines composed with less T-cell epitopes, DNA vaccine pVAX1-m1-m2-s1-s2 containing 4 fragments with concentrated T-epitopes provided the highest ACI of 180.39. DNA vaccines composed of antigens from two developmental stages were more effective than the single-stage ones. Especially DNA vaccine pVAX1-m1-m2-s1-s2 provided the most effective protection with the ACI of 18039. Furthermore, cytokines IL-2 or IFN-gamma could improve the efficacy of the multi-epitope DNA vaccines significantly. Overall, pVAX1-m1-m2-s1-s2-IFN-gamma provided the most effective protection with the ACI of 189.92. The multi-epitope DNA vaccines revealed in this study provide new candidates for Eimeria vaccine development. (C) 2015 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Efficacy of chimeric DNA vaccines encoding Eimeria tenella 5401 and chicken IFN-gamma or IL-2 against coccidiosis in chickens. Song, Xiaokai,Huang, Xinmei,Yan, Ruofeng,Xu, Lixin,Li, Xiangrui,Huang, Xinmei.

[2]The preparation of gypenosides liposomes and its effects on the peritoneal macrophages function in vitro. Yu, Yun,Bo, Ruonan,Huang, Yee,Hu, Yuanliang,Liu, Jiaguo,Wu, Yi,Tao, Yang,Wang, Deyun,Lu, Yu.

[3]Effect of epimedium polysaccharide-propolis flavone immunopotentiator on immunosuppression induced by cyclophosphamide in chickens. Fan, Yunpeng,Wang, Deyun,Liu, Jiaguo,Zhao, Xiaojuan,Luong Nguyen,Hu, Yuanliang,Fan, Yunpeng,Song, Xiaoping,Zhang, Weimin,Lu, Yu.

[4]Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus. Du, Luping,Pang, Fengjiao,Xu, Xiangwei,Huang, Kehe,Du, Luping,Li, Bin,Pang, Fengjiao,Yu, Zhengyu,Xu, Xiangwei,Fan, Baochao,Tan, Yeping,He, Kongwang,Du, Luping,Li, Bin,Yu, Zhengyu,Fan, Baochao,Tan, Yeping,He, Kongwang.

[5]The swine CD81 enhances E2-based DNA vaccination against classical swine fever. Li, Wenliang,Zhou, Bin,Liu, Xia,Yang, Leilei,Zhang, Wenwen,Jiang, Jieyuan,Zhou, Bin.

[6]Targeted Delivery of GP5 Antigen of PRRSV to M Cells Enhances the Antigen-Specific Systemic and Mucosal Immune Responses. Du, Luping,Yu, Zhengyu,Pang, Fengjiao,Xu, Xiangwei,Mao, Aihua,He, Kongwang,Li, Bin,Du, Luping,Yu, Zhengyu,Pang, Fengjiao,Xu, Xiangwei,Mao, Aihua,He, Kongwang,Li, Bin,Du, Luping,Yuan, Wanzhe. 2018

[7]Construction and evaluation of the eukaryotic expression plasmid encoding two copies of somatostatin genes fused with hepatitis B surface antigen gene S. Liang, Aixin,Han, Li,Yao, Yanfeng,Moaeen-ud-Din, M.,Yang, Liguo,Cao, Shaoxian. 2008

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台