您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Expression profiles and interaction suggest TBK1 can be regulated by Nrdp1 in response to immune stimulation in large yellow croaker Larimichthys crocea

文献类型： 外文期刊

作者： Zhang, Dong Ling 1 ; Yu, Da Hui 2 ; Chen, Jian 1 ; Fan, Sigang 2 ; Wang, Zhi Yong 1 ;

作者机构： 1.Jimei Univ, Coll Fisheries, Key Lab Hlth Mariculture East China Sea, Minist Agr, Xiamen 361021, Fujian Province, Peoples R China

2.Chinese Acad Fishery Sci, South China Sea Resource Exploitat & Protect Coll, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

关键词： Larimichthys crocea;TBK1;Nrdp1;Protein interaction;Immune stimulation

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： TBK1 has been extensively studied in mammals because of its important roles as a molecular bridge, linking the TLRs (TLR3 and TLR4) and RLRs signals to activate transcriptional factors IRF3 and IRF7 for IFN-I production. However, the information on molecular and functional characteristics of TBK1 in teleosts is limited. In this study, the molecular characterization and immune response of TBK1 in Larimichthys crocea (named as LcTBK1) as well as its interaction with Nrdp1 were investigated. Sequence analysis demonstrated that LcTBK1 included four functional motifs, the N-terminal protein kinase domain and ATP-binding site, middle ULD and C-terminal coiled-coil domain. The tissue expression profiles indicated that LcTBK1 gene was constitutively expressed in the twelve tissues examined, with high expression in brain. Temporal expression analysis showed that LcTBK1 mRNA was obviously increased in the liver after injection of LPS, Poly I:C and inactive Vibrio parahaemolyticus, however, declined at some time points in spleen and head-kidney. Furthermore, we found that LcTBK1 can interact with LcNrdp1, an E3 ubiquitin ligase that involved in immune response to Cryptoccuyon irritans infection in L. crocea. The qPCR showed that LcNrdp1 was also significantly up-regulated in liver, down-regualted at some time points in spleen and head-kidney after LPS, Poly I:C and inactive V. parahaemolyticus injection, although the expression patterns of the two genes after the three treatments were different in change magnitude and up-regulation timespan. These results suggested that LcTBK1 was involved in L. crocea defense against the pathogen infection and can be regulated by Nrdp1 in PPRs signaling pathway of fishes. (C) 2015 Elsevier Ltd. Alt rights reserved.

  • 相关文献

[1]Characterization of E3 ubiquitin ligase neuregulin receptor degradation protein-1 (Nrdp1) in the large yellow croaker (Larimichthys crocea) and its immune responses to Cryptocaryon irritans. Zhang, Dong Ling,Han, Fang,Xiao, Shi Jun,Chen, Jian,Wang, Zhi Yong,Yu, Da Hui,Li, Ming Yun.

[2]Anti-parasitic effects of Leptomycin B isolated from Streptomyces sp CJK17 on marine fish ciliate Cryptocaryon irritans. Yin, Fei,Sun, Peng,Tang, Baojun,Gong, Hui,Ke, Qiaozhen,Li, Anxing.

[3]Characterisation and expression analysis of two terminal complement components: C7 and C9 from large yellow croaker, Larimichthys crocea. Guo, Baoying,Wu, Changwen,Lv, Zhenming,Liu, Changlin.

[4]Mass mortality associated with a viral-induced anaemia in cage-reared large yellow croaker, Larimichthys crocea (Richardson). Xu, W. J.,Shi, H.,Xie, J. J.,Wang, G. S.,Qin, Z. D.,Zhou, Y.,Liu, X. L.,Asim, M.,Lin, L.,Jiang, N.,Zeng, L. B.,Wang, W. M.,Lin, L.,Lin, L.,Lin, L..

[5]Molecular cloning and expression analysis of scd1 gene from large yellow croaker Larimichthys crocea under cold stress. Xu, Hao,Zhang, Dong Ling,Lv, Chang Huan,Luo, Hui Yu,Wang, Zhi Yong,Yu, Da Hui.

[6]Transcriptome and analysis on the complement and coagulation cascades pathway of large yellow croaker (Larimichthys crocea) to ciliate ectoparasite Cryptocaryon irritans infection. Yin, Fei,Gao, Quanxin,Tang, Baojun,Sun, Peng,Han, Kunhuang,Huang, Weiqing,Han, Kunhuang,Huang, Weiqing.

[7]Characterization and expression analysis of BCA2 gene in large yellow croaker, Larimichthys crocea. Zhang, Dong Ling,Chen, Jian,Wang, Zhi Yong,Yu, Da Hui.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台